Progress in Molecular Characterization and Epitope Identification of Major Buckwheat Allergens

QIN Yufei; YU Ning; KANG Wenhan; LI Yang; HU Fengxin; ZHANG Jiukai; CHEN Ying

doi:10.13386/j.issn1002-0306.2024030468

Volume 46 Issue 5

Feb. 2025

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2025 > 46(5): 397-404. > DOI: 10.13386/j.issn1002-0306.2024030468

QIN Yufei, YU Ning, KANG Wenhan, et al. Progress in Molecular Characterization and Epitope Identification of Major Buckwheat Allergens[J]. Science and Technology of Food Industry, 2025, 46(5): 397−404. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030468.

Citation:

PDF (2034 KB)

Progress in Molecular Characterization and Epitope Identification of Major Buckwheat Allergens

QIN Yufei^{1, 2,},
YU Ning¹,
KANG Wenhan¹,
LI Yang^{1, 3},
HU Fengxin^{1, 4},
ZHANG Jiukai¹,
CHEN Ying^1, ,

1.
Chinese Academy of Inspection and Quarantine, Beijing 100176, China
2.
College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210046, China
3.
College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China
4.
School of Food and Health, Beijing Technology and Business University, Beijing 100048, China

More Information

Received Date: March 28, 2024
Available Online: January 01, 2025

Graphical Abstract

Abstract

Abstract

Buckwheat is a common food allergen that can cause diseases related to the respiratory, digestive, and circulatory systems, and in severe cases, it can lead to anaphylactic shock or even death. Identifying the main allergenic proteins in buckwheat and determining their allergenic epitopes is of great significance for understanding the mechanism of buckwheat allergy and for the prevention and treatment of related allergic diseases. This paper reviews the structural characteristics and research progress on the epitopes of the main buckwheat allergens (Fag e 1, Fag e 2, Fag e 3, Fag e 4, Fag e 5, Fag t 2, Fag t 3, Fag t 6), aiming to provide a reference for in-depth research and prevention of buckwheat allergies.
- buckwheat allergens,
- structural characteristic,
- epitope identification,
- allergenicity

FullText(HTML)

References (63)

References

[1]	ZHU F. Buckwheat proteins and peptides:Biological functions and food applications[J]. Trends in Food Science & Technology,2021,110:155−167.
[2]	SOFI S A, AHMED N, FAROOQ A, et al. Nutritional and bioactive characteristics of buckwheat, and its potential for developing gluten-free products:An updated overview[J]. Food Science & Nutrition,2023,11(5):2256−2276.
[3]	NORBÄCK D, WIESLANDER G. A review on epidemiological and clinical studies on buckwheat allergy[J]. Plants-Basel,2021,10(3):607. doi: 10.3390/plants10030607
[4]	赵霞, 韩一军, 姜利娜, 等. 我国苦荞市场与产业调查分析报告[J]. 农产品市场,2021(15):42−44. [ZHAO Xia, HAN Yijun, JIANG Lina, et al. China's buckwheat market and industry research and analysis report[J]. Agricultural Market,2021(15):42−44.] ZHAO Xia, HAN Yijun, JIANG Lina, et al. China's buckwheat market and industry research and analysis report[J]. Agricultural Market, 2021（15）: 42−44.
[5]	KIM S A, TOUSHIK S H, LEE J E, et al. Ultrasensitive monoclonal antibodies specific to thermal stable-soluble proteins of buckwheat[J]. Food Chemistry,2023,423:136269. doi: 10.1016/j.foodchem.2023.136269
[6]	MIYAMOTO M, KATO M, YOSHIHARA S, et al. Food protein-induced enterocolitis syndrome due to buckwheat:A case report[J]. Allergologia et Immunopathologia,2023,51(3):25−27. doi: 10.15586/aei.v51i3.826
[7]	OLIVIERI B, SKYPALA I J. New arrivals in anaphylaxis to foods[J]. Current Opinion in Allergy and Clinical Immunology,2023,23(5):357−363. doi: 10.1097/ACI.0000000000000936
[8]	ERQUICI S P, ABRAIRA M B, ARENAS S D, et al. Rhinoconjunctivitis and occupational asthma due to buckwheat flour allergy[J]. Archivos de Bronconeumologia,2020,56(7):466. doi: 10.1016/j.arbr.2020.02.004
[9]	JUNGEWELTER S, AIRAKSINEN L, PESONEN M. Occupational buckwheat allergy as a cause of allergic rhinitis, asthma, contact urticaria and anaphylaxis-An emerging problem in food-handling occupations?[J]. American Journal of Industrial Medicine,2020,63(11):1047−1053. doi: 10.1002/ajim.23185
[10]	FAROOQ M, WANI S M, MIR S A, et al. An overview of buckwheat allergy:A rare allergenic food[J]. Journal of Food Composition and Analysis,2023,123:105616. doi: 10.1016/j.jfca.2023.105616
[11]	JUNGEWELTER S, SUOMELA S, AIRAKSINEN L. Occupational IgE-mediated psyllium allergy in contemporary gluten-free and vegan baking:A case of allergic rhinitis[J]. American Journal of Industrial Medicine,2021,64(5):431. doi: 10.1002/ajim.23238
[12]	SUDHARSON S, KALIC T, HAFNER C, et al. Newly defined allergens in the WHO/IUIS Allergen Nomenclature Database during 01/2019-03/2021[J]. Allergy,2021,76(11):3359−3373. doi: 10.1111/all.15021
[13]	ZHANG Z Y, LI X M, WANG H, et al. Seafood allergy:Allergen, epitope mapping and immunotherapy strategy[J]. Critical Reviews in Food Science and Nutrition,2023,63(10):1314−1338. doi: 10.1080/10408398.2023.2181755
[14]	LEE A S E, SUPRUN M, SAMPSON H. Epitope-based IgE assays and their role in providing diagnosis and prognosis of food allergy[J]. Journal of Allergy and Clinical Immunology-in Practice,2023,11(10):2983−2988. doi: 10.1016/j.jaip.2023.06.043
[15]	PLATTS-MILLS T A, HILGER C, JAPPE U, et al. Carbohydrate epitopes currently recognized as targets for IgE antibodies[J]. Allergy,2021,76(8):2383−2394. doi: 10.1111/all.14802
[16]	MATSUMOTO R, FUJINO K, NAGATA Y, et al. Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic patients' IgE[J]. Allergy,2004,59(5):533−538. doi: 10.1046/j.1398-9995.2003.00412.x
[17]	张润敏, 刘海宁, 姚慧鹏, 等. 苦荞过敏蛋白TBW17基因克隆及其抗原表位分析[J]. 基因组学与应用生物学,2016,35(12):3481−3486. [ZHANG Runmin, LIU Haining, YAO Huipeng, et al. Cloning of buckwheat allergenic protein TBW17 gene and its antigenic epitope analysis[J]. Genomics and Applied Biology,2016,35(12):3481−3486.] ZHANG Runmin, LIU Haining, YAO Huipeng, et al. Cloning of buckwheat allergenic protein TBW17 gene and its antigenic epitope analysis[J]. Genomics and Applied Biology, 2016, 35（12）: 3481−3486.
[18]	侯晓军. 苦荞过敏蛋白TB22kDa的cDNA片断克隆、原核表达及初步纯化[D]. 太原:山西大学, 2003. [HOU Xiaojun. Cloning, prokaryotic expression and preliminary purification of cDNA fragment of buckwheat allergenic protein TB22kDa[D]. Taiyuan:Shanxi University, 2003.] HOU Xiaojun. Cloning, prokaryotic expression and preliminary purification of cDNA fragment of buckwheat allergenic protein TB22kDa[D]. Taiyuan: Shanxi University, 2003.
[19]	CUI X D, TIAN W H, WANG W H, et al. Sequence analysis and biochemical characteristics of two non-specific lipid transfer proteins from tartary buckwheat seeds[J]. Protein and Peptide Letters,2023,30(6):520−529. doi: 10.2174/0929866530666230511154511
[20]	李俊慧, 邵军军, 常惠芸, 等. 抗原表位鉴定方法的研究进展[J]. 中国兽医科学,2021,51(6):678−683. [LI Junhui, SHAO Junjun, CHANG Huiyun, et al. Advances in antigenic epitope identification methods[J]. Chinese Veterinary Science,2021,51(6):678−683.] LI Junhui, SHAO Junjun, CHANG Huiyun, et al. Advances in antigenic epitope identification methods[J]. Chinese Veterinary Science, 2021, 51（6）: 678−683.
[21]	PARK J Y, CHO S H. Production of monoclonal antibody of heat-labile toxin A subunit to identify enterotoxigenic Escherichia coli by epitope mapping using synthetic peptides[J]. Frontiers in Immunology,2023,14:1152910. doi: 10.3389/fimmu.2023.1152910
[22]	DANG X B, GUELEN L, HULSIK D L, et al. Epitope mapping of monoclonal antibodies:A comprehensive comparison of different technologies[J]. Mabs,2023,15(1):2285285. doi: 10.1080/19420862.2023.2285285
[23]	JIA B X, OJIMA-KATO T, KOJIMA T, et al. Rapid and cost-effective epitope mapping using pure ribosome display coupled with next-generation sequencing and bioinformatics[J]. Journal of Bioscience and Bioengineering,2024,137(4):321−328. doi: 10.1016/j.jbiosc.2024.01.008
[24]	JAROSZEWICZ W, MORCINEK-ORLOWSKA J, PIERZYNOWSKA K, et al. Phage display and other peptide display technologies[J]. Fems Microbiology Reviews,2022,46(2):fuab052. doi: 10.1093/femsre/fuab052
[25]	ZHANG A L, ZHAO H J, PEI S H, et al. Identification and structure of epitopes on cashew allergens Ana o 2 and Ana o 3 using phage display[J]. Molecules,2023,28(4):1880. doi: 10.3390/molecules28041880
[26]	URISU A, KONDO Y, MORITA Y, et al. Isolation and characterization of a major allergen in buckwheat seeds[J]. Current Advances in Buckwheat Research, 1995:965-974.
[27]	TANAKA K, MATSUMOTO K, AKASAWA A, et al. Pepsin-resistant 16-kD buckwheat protein is associated with immediate hypersensitivity reaction in patients with buckwheat allergy[J]. Journal of Innate Immunity,2002,129(1):49−56.
[28]	CHOI S Y, SOHN J H, LEE Y W, et al. Characterization of buckwheat 19-kD allergen and its application for diagnosing clinical reactivity[J]. International Archives of Allergy and Immunology,2007,144(4):267−274. doi: 10.1159/000106315
[29]	FUJIMURA M, MINAMI Y, WATANABE K, et al. Purification, characterization, and sequencing of a novel type of antimicrobial peptides, Fa-AMP1 and Fa-AMP2, from seeds of buckwheat (Fagopyrum esculentum Moench.)[J]. Bioscience, Biotechnology, and Biochemistry,2003,67(8):1636−1642. doi: 10.1271/bbb.67.1636
[30]	GEISELHART S, NAGL C, DUBIELA P, et al. Concomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy[J]. Clinical & Experimental Allergy,2018,48(2):217−224.
[31]	HU F, YE Z Y, DONG K, et al. Divergent structures and functions of the cupin proteins in plants[J]. International Journal of Biological Macromolecules,2023,242:124791. doi: 10.1016/j.ijbiomac.2023.124791
[32]	SANO M, NAKAGAWA M, OISHI A, et al. Diversification of 13S globulins, allergenic seed storage proteins, of common buckwheat[J]. Food Chemistry,2014,155:192−198. doi: 10.1016/j.foodchem.2014.01.047
[33]	ZHOU Y, OUYANG B, DU L, et al. Effects of ultra-high-pressure treatment on the structural and functional properties of buckwheat 13S globulin[J]. Food Science and Human Wellness,2022,11(4):895−903. doi: 10.1016/j.fshw.2022.03.008
[34]	CHNAPEK M, GÁLOVÁ Z, BALÁZOVÁ Z, et al. Application of two-dimensional electrophoresis and mass spectrometry for the detection of allergens in selected varieties of wheat, oats and buckwheat[J]. Journal of Microbiology Biotechnology and Food Sciences,2023,13(1):e9934. doi: 10.55251/jmbfs.9934
[35]	MARUYAMA FUNSTSUKI W, FUJINO K, SUZUKI T, et al. Quantification of a major allergenic protein in common buckwheat cultivars by an enzyme-linked immunosorbent assay (ELISA)[J]. Fagopyrum,2004,21:39−44.
[36]	YOSHIOKA H, OHMOTO T, URISU A, et al. Expression and epitope analysis of the major allergenic protein Fag e 1 from buckwheat[J]. 2004, 161(7):761-767.
[37]	YANAGIDA N, SATO S, MARUYAMA N, et al. Specific IgE for Fag e 3 predicts oral buckwheat food challenge test results and anaphylaxis:A pilot study[J]. International Archives of Allergy and Immunology,2018,176(1):8−14. doi: 10.1159/000487135
[38]	SOUZA P F. The forgotten 2S albumin proteins:Importance, structure, and biotechnological application in agriculture and human health[J]. International Journal of Biological Macromolecules,2020,164:4638−4649. doi: 10.1016/j.ijbiomac.2020.09.049
[39]	BUENO D C, MARTÍN P L, PARRÓN J, et al. Characterization of relevant biomarkers for the diagnosis of food allergies:An overview of the 2S albumin family[J]. Foods,2021,10(6):1235. doi: 10.3390/foods10061235
[40]	CHOI S Y, SOHN J H, LEE Y W, et al. Application of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity[J]. Annals of Allergy, Asthma & Immunology, 2007, 99(3):254−260.
[41]	SATOH R, KOYANO S, TAKAGI K, et al. Identification of an IgE-binding epitope of a major buckwheat allergen, BWp16, by SPOTs assay and mimotope screening[J]. International Archives of Allergy and Immunology,2010,153(2):133−140. doi: 10.1159/000312630
[42]	DI SOMMA A, MORETTA A, CANÈ C, et al. Antimicrobial and antibiofilm peptides[J]. Biomolecules,2020,10(4):652. doi: 10.3390/biom10040652
[43]	SATHOFF A E, LEWENZA S, SAMAC D A. Plant defensin antibacterial mode of action against Pseudomonas species[J]. Bmc Microbiology,2020,20(1):1−11. doi: 10.1186/s12866-019-1672-7
[44]	BROEKAERT W F, CAMMUE B P, DE BOLLE M F, et al. Antimicrobial peptides from plants[J]. Critical Reviews in Plant Sciences,1997,16(3):297−323. doi: 10.1080/07352689709701952
[45]	GEISELHART S, NAGL C, DUBIELA P, et al. Concomitant sensitization to legumin, Fag e 2 and Fag e 5 predicts buckwheat allergy[J]. 2018, 48(2):217−224.
[46]	LUTHAR Z, GOLOB A, GERM M, et al. Tartary buckwheat in human nutrition[J]. Plants-Basel,2021,10(4):700. doi: 10.3390/plants10040700
[47]	KOYANO S, TAKAGI K, TESHIMA R, et al. Molecular cloning of cDNA, recombinant protein expression and characterization of a buckwheat 16-kDa major allergen[J]. International Archives of Allergy and Immunology,2006,140(1):73−81. doi: 10.1159/000092038
[48]	ZHANG X, YUAN J M, CUI X D, et al. Molecular cloning, recombinant expression, and immunological characterization of a novel allergen from tartary buckwheat[J]. Journal of Agricultural and Food Chemistry,2008,56(22):10947−10953. doi: 10.1021/jf801855a
[49]	CHEN F, LI H, FAN X, et al. Identification of a novel major allergen in buckwheat seeds:Fag t 6[J]. Journal of Agricultural and Food Chemistry,2021,69(45):13315−13322. doi: 10.1021/acs.jafc.1c01537
[50]	郑蓓. 苦荞16 kDa过敏原Fag t 2结构与功能研究[D]. 杨凌:西北农林科技大学, 2021. [ZHEN Pei. Structure and function of the buckwheat 16 kDa allergen, Fag t 2[D]. Yangling:North West Agriculture and Forestry University, 2021.] ZHEN Pei. Structure and function of the buckwheat 16 kDa allergen, Fag t 2[D]. Yangling: North West Agriculture and Forestry University, 2021.
[51]	CHEN P, GUO Y, YAN Q, et al. Molecular cloning and characterization of Fag t 2:A 16‐kDa major allergen from Tartary buckwheat seeds[J]. Allergy,2011,66(10):1393−1395. doi: 10.1111/j.1398-9995.2011.02657.x
[52]	ZHENG B, ZHANG H, SHEN W, et al. Core epitope analysis of 16 kDa allergen from tartary buckwheat[J]. Food Chemistry,2021,346:128953. doi: 10.1016/j.foodchem.2020.128953
[53]	贺东亮, 张政, 任晓霞, 等. 苦荞过敏原TBa和TBb基因的共表达及其包涵体复性的研究[J]. 中国农学通报,2009,25(8):50−52. [HE Dongliang, ZHANG Zheng, REN Xiaoxia, et al. Co-expression of TBa and TBb genes of buckwheat allergens and their inclusion body complexes[J]. China Agronomy Bulletin,2009,25(8):50−52.] HE Dongliang, ZHANG Zheng, REN Xiaoxia, et al. Co-expression of TBa and TBb genes of buckwheat allergens and their inclusion body complexes[J]. China Agronomy Bulletin, 2009, 25（8）: 50−52.
[54]	YANG Z, LI Y, LI C, et al. Synthesis of hypoallergenic derivatives of the major allergen Fag t 1 from tartary buckwheat via sequence restructuring[J]. Food and Chemical Toxicology,2012,50(8):2675−2680. doi: 10.1016/j.fct.2012.03.039
[55]	REN X, ZHANG X, LI Y, et al. Epitope mapping and immunological characterization of a major allergen TBa in tartary buckwheat[J]. Biotechnology Letters,2010,32:1317−1324. doi: 10.1007/s10529-010-0281-1
[56]	赵小珍, 张政, 景巍, 等. 苦荞麦主要过敏蛋白N端基因片段的克隆及序列分析[J]. 食品科学,2006(10):41−44. [ZHAO Xiaozhen, ZHANG Zheng, JING Wei, et al. Cloning and sequence analysis of the N-terminal gene fragment of buckwheat major allergenic protein[J]. Food Science,2006(10):41−44.] ZHAO Xiaozhen, ZHANG Zheng, JING Wei, et al. Cloning and sequence analysis of the N-terminal gene fragment of buckwheat major allergenic protein[J]. Food Science, 2006（10）: 41−44.
[57]	贺东亮, 崔晓东, 赵小珍, 等. 过敏蛋白TBb的免疫活性鉴定及其表位预测[J]. 免疫学杂志,2009,25(2):137−140,144. [HE Dongliang, CUI Xiaodong, ZHAO Xiaozhen, et al. Identification of immunoreactivity of the allergenic protein TBb and its epitope prediction[J]. Journal of Immunology,2009,25(2):137−140,144.] HE Dongliang, CUI Xiaodong, ZHAO Xiaozhen, et al. Identification of immunoreactivity of the allergenic protein TBb and its epitope prediction[J]. Journal of Immunology, 2009, 25（2）: 137−140,144.
[58]	LI P, CUI X, LI Y, et al. Epitope mapping and identification on a 3D model built for the tartary buckwheat allergic protein TBb[J]. Acta Biochim Biophys Sin,2011,43(6):441−447. doi: 10.1093/abbs/gmr036
[59]	BOARD A J, CROWTHER J M, ACEVEDO-FANI A, et al. How plants solubilise seed fats:Revisiting oleosin structure and function to inform commercial applications[J]. Biophysical Reviews,2022,14(1):257−266. doi: 10.1007/s12551-021-00923-5
[60]	HE X R, YANG Y, CHEN Y X, et al. Immunoglobulin e epitope mapping and structure-allergenicity relationship analysis of crab allergen scy p 9[J]. Journal of Agricultural and Food Chemistry,2023,71(45):17379−17390.
[61]	TAHIR S, BOURQUARD T, MUSNIER A, et al. Accurate determination of epitope for antibodies with unknown 3D structures[J]. Mabs,2021,13(1):1961349. doi: 10.1080/19420862.2021.1961349
[62]	MARINI-RAPOPORT O, FERNÁNDEZ-QUINTERO M L, KESWANI T, et al. Defining the cross-reactivity between peanut allergens Ara h 2 and Ara h 6 using monoclonal antibodies[J]. Clinical and Experimental Immunology,2024,216(1):25−35. doi: 10.1093/cei/uxae005
[63]	POM A, SMITH S A, CHRUSZCZ M, et al. Precision engineering for localization, validation, and modification of allergenic epitopes[J]. Journal of Allergy and Clinical Immunology,2024,153(3):560−571. doi: 10.1016/j.jaci.2023.12.017

Supplements (0)

Get Citation

PDF

XML

Article Metrics

Article views (24) PDF downloads (7)

Science and Technology of Food Industry

Progress in Molecular Characterization and Epitope Identification of Major Buckwheat Allergens

Abstract

References

Catalog

Article Metrics

Export File

Citation

Format

Content