Progress on Synthetic Biology For Steviol Glycosides Biosynthesis

LIU Mengyu; HE Hailin; MA Ting; SU Xiaochun; DING Yifei; WANG Jinxuan; LEI Yinfeng; LI Shubo

doi:10.13386/j.issn1002-0306.2024030397

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LIU Mengyu, HE Hailin, MA Ting, et al. Progress on Synthetic Biology For Steviol Glycosides Biosynthesis[J]. Science and Technology of Food Industry, 2025, 46(6): 1−11. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030397.

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Progress on Synthetic Biology For Steviol Glycosides Biosynthesis

1.
College of Light industry and Food Engineering, Guangxi University, Nanning 530004, China
2.
Key Laboratory of Deep Processing and Safety Control for Specialty Agricultural Products in Guangxi Universities, Education Department of Guangxi Zhuang Autonomous Region, Nanning 530004, China

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Received Date: March 25, 2024
Available Online: January 10, 2025

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Abstract

Abstract

Stevioside, also known as stevia, is a class of diterpenoids extracted from stevia with high sweetness, low calories, safety and non-toxicity, hypoglycemic, hypotensive, and other physiological characteristics. It is regarded as the "world's third natural glycogen," and widely used in the food, pharmaceutical, daily-use chemicals, brewing, and other industries. However, extracts based on the traditional extraction process have ^[1]problems such as difficult purification, low extraction efficiency, and excessive consumption of solvents, which limit their value and application scope. The rapid development of synthetic biology provides a new green and efficient production mode for the production of plant-based natural products. To this end, the article reviews the research progress of steviol glycosides' biosynthesis based on the elucidation of their structure and physiological activities. It analyzes the biosynthetic pathways of steviol glycosides and the catalytic mechanisms of the key enzymes involved. The focus is on the mechanisms and approaches for biocatalytic synthesis using glycosyltransferases and the de novo synthesis of specific steviol glycosides by microorganisms. Additionally, it discusses the application of two biosynthetic methods in the production of high sweetness, low bitterness rebaudiosides A, D, and M. Based on the aforementioned research advances, the paper explores the main challenges currently faced in the biosynthesis of steviol glycosides and future research directions, aiming to provide theoretical insights for the biosynthesis of steviol glycosides.
- steviol glycosides,
- synthetic pathway,
- glycosyltransferases,
- biocatalysis,
- biosynthesis

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Science and Technology of Food Industry

Progress on Synthetic Biology For Steviol Glycosides Biosynthesis

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