Effect of Gardenia Yellow Pigment on Dextran Sodium Sulfate-induced Colitis in Rats and Its Effect on Intestinal Flora

LIU Lijun; ZHAN Jiahao; SHUAI Jinhao; JIANG Shenhua; CHEN Si; LIU Changjin; ZHANG Ailin; HAO Shu

doi:10.13386/j.issn1002-0306.2024030188

Volume 46 Issue 5

Feb. 2025

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Science and Technology of Food Industry > 2025 > 46(5): 344-354. > DOI: 10.13386/j.issn1002-0306.2024030188

LIU Lijun, ZHAN Jiahao, SHUAI Jinhao, et al. Effect of Gardenia Yellow Pigment on Dextran Sodium Sulfate-induced Colitis in Rats and Its Effect on Intestinal Flora[J]. Science and Technology of Food Industry, 2025, 46(5): 344−354. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024030188.

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Effect of Gardenia Yellow Pigment on Dextran Sodium Sulfate-induced Colitis in Rats and Its Effect on Intestinal Flora

1.
College of Food Science and Bioengineering, Tianjin Agricultural University, Tianjin 300384, China
2.
School of Pharmacy and Life Science, Jiujiang University, Jiujiang Key Laboratory of Food Processing and Safety, Jiujiang 332000, China
3.
College of Food Science and Technology, State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, China

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Received Date: March 13, 2024
Available Online: January 01, 2025

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Abstract

Abstract

Objective: To explore the effect of gardenia yellow pigment (GYP) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in rats. Methods: Four groups of rats were established: Normal group, model group, positive control group, and GYP intervention group. The health status of the rats was recorded on the day of sampling, and the disease activity index (DAI) score was calculated. The weight change, DAI score, colon tissue morphology, short chain fatty acids (SCFAs) content and the composition of intestinal flora were compared between the four groups. Furthermore, preliminary analysis was conducted on the mechanism of GYP in alleviating UC in rats through network pharmacology and molecular docking. Results: The results indicated that GYP not only effectively alleviated the symptoms of ulcerative colitis such as body mass loss, hematochezia, diarrhea, and the shortening of colon length and colon tissue injury in rats. Moreover, GYP significantly increased the relative abundance of Firmicutes and Actinobacteria, as well as the content of acetic acid (P<0.05), and significantly reduced the relative abundance of Bacteroidetes and Spirochetes (P<0.01). Through network pharmacology screening, crocetin was identified as a key component for alleviating UC in GYP, and 38 intersection targets and 86 KEGG pathways were identified. In addition, molecular docking results demonstrated that crocetin had good affinity for core targets for alleviating UC, among which ACE had the highest binding degree. Conclusion: GYP may alleviate intestinal damage and microbial disruption in DSS induced UC rats by increasing the content of short chain fatty acids and maintaining the homeostasis of gut microbiota.
- gardenia yellow pigment,
- ulcerative colitis,
- network pharmacology,
- molecular docking,
- short-chain fatty acids,
- intestinal flora

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References

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Effect of Gardenia Yellow Pigment on Dextran Sodium Sulfate-induced Colitis in Rats and Its Effect on Intestinal Flora

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