Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

LI Qian; ZENG Xiangbing; SUN Xitong; CHEN Xiaoyi; LI Miao; WANG Tianyu; HUANG Fan; SUN Fanghong; LI Xianzhen

doi:10.13386/j.issn1002-0306.2024020096

Volume 46 Issue 5

Feb. 2025

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Science and Technology of Food Industry > 2025 > 46(5): 168-177. > DOI: 10.13386/j.issn1002-0306.2024020096

LI Qian, ZENG Xiangbing, SUN Xitong, et al. Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41[J]. Science and Technology of Food Industry, 2025, 46(5): 168−177. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2024020096.

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Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

1.
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, China
2.
School of Light Industry and Chemical Engineering, Dalian Polytechnic University, Dalian 116034, China

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Received Date: February 19, 2024
Available Online: December 24, 2024

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Abstract

In order to construct a new catalytic system of immobilized lipase and improve the stability of enzyme, the phenol amino modified ordered mesoporous material (MCM-41) was introduced for the study of lipase immobilization. The immobilized enzyme was also applied to the actual reaction system. The optimized conditions of lipase immobilization were as follows: Immobilization temperature of 30 ℃, immobilization pH of 8.0, immobilization time of 7 h, initial enzyme concentration of 4 mg/mL, and the enzyme loading was 26.40 mg/g. Meanwhile, the optimum reaction temperature of the immobilized enzyme was 50 ℃, and the optimum reaction pH was 7.0, together with the highest enzyme activity was 4108±34.74 U/g carrier. The immobilized enzyme was recycled for eight times, and it retained 54.70% of the initial enzyme activity. While after 30 days of storage at 4 ℃, it still kept 71.10% of the initial enzyme activity. In addition, the immobilized enzyme was used to catalyze the synthesis of hexyl laurate, and the highest conversion of lauric acid was 65.38%. It was indicated that the lipase immobilized on phenol amino modified MCM-41 exhibited high enzyme activity and good stability. When it was applied in the actual catalytic reaction, the immobilized enzyme displayed high catalytic efficiency. Furthermore, it was proved that phenol amino modification was a green and effective modification strategy, which could provide ideas for further application of phenol amino coating.
- phenol amino modification,
- MCM-41,
- immobilized lipase,
- process optimization,
- synthesis of hexyl laurate

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Process Optimization and Application of Lipase Immobilized on Phenol Amino Modified MCM-41

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