Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of <i>Salmonella</i> Typhimurium

WANG Yanyan; XIAO Xingning; LI Jian; MA Jiele; DONG Xiaoping; YI Zhengkai; LOU Qiaoming; WANG Wen

doi:10.13386/j.issn1002-0306.2023120142

Volume 45 Issue 22

Nov. 2024

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Science and Technology of Food Industry > 2024 > 45(22): 208-216. > DOI: 10.13386/j.issn1002-0306.2023120142

WANG Yanyan, XIAO Xingning, LI Jian, et al. Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium[J]. Science and Technology of Food Industry, 2024, 45(22): 208−216. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2023120142.

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Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium

1.
School of Food Science and Engineering, Ningbo University, Ningbo 315800, China
2.
Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310012, China
3.
School of Materials Science and Engineering, Zhejiang Sci-Tech University, Hangzhou 310021, China

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Received Date: December 13, 2023
Available Online: September 09, 2024

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Abstract

Objective: To investigate the bactericidal mechanism of a composite sol made of carbon nitride and chitosan on Salmonella typhimurium by studying its effects on lipid metabolism. Methods: Transmission electron microscopy and protein leakage concentration were utilized to evaluate the magnitude of cell membrane damage induced by carbon nitride-chitosan composite sol at a cellular level. In addition, lipid metabolomics was employed to investigate the bactericidal process by examining several metabolite functions and regulatory networks associated with bacterial lipid metabolism. Results: The composite sol of carbon nitride-chitosan caused more extensive damage to the cell membrane of Salmonella typhimurium. In lipid metabolism, the significantly up-regulated in 203 metabolites and down-regulated in 95 metabolites. These metabolites were particularly involved in the metabolism of fatty acids, glycerophospholipids, and glycolipids. Metabolite function analysis showed that the composite sol treatment disrupted various metabolic pathways in bacteria. These pathways included fatty acid biosynthesis, glycerol phospholipid metabolism, linoleic acid metabolism, tetraenoic acid metabolism, as well as pathways related to cell energy metabolism, material transport, and signal conduction. These disturbances finally resulted in the demise of the bacteria. Conclusion: This study revealed from the perspective of lipid metabolism that the combination of carbon nitride and chitosan disrupts the metabolic pathways of Salmonella, especially those related to lipid metabolism, leading to bacterial metabolic disruption and death. It provides a theoretical basis for comprehending the mechanisms by which photocatalytic materials exert their antibacterial properties.
- Salmonella,
- targeted lipid metabolomics,
- graphene carbon nitride,
- chitosan,
- photocatalysis

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References

[1]	尹文琴, 李瑞锐. 一起鼠伤寒沙门菌感染引起的食物中毒流行病学调查报告[J]. 食品安全导刊,2023(27):25−27,37. [YIN W Q, LI R R. Report of an epidemiologic investigation of food poisoning caused by Salmonella typhimurium infection[J]. Food Safety Guide,2023(27):25−27,37.] YIN W Q, LI R R. Report of an epidemiologic investigation of food poisoning caused by Salmonella typhimurium infection[J]. Food Safety Guide, 2023（27）: 25−27,37.
[2]	吴宪. 我国食品沙门氏菌污染率与引起的发病率统计分析[D]. 大连:大连理工大学, 2021. [WU X. Statistical analysis of the contamination rate and morbidity caused by Salmonella in food in China[D]. Dalian:Dalian University of Technology, 2021.] WU X. Statistical analysis of the contamination rate and morbidity caused by Salmonella in food in China[D]. Dalian: Dalian University of Technology, 2021.
[3]	陈崟珺. 食品微生物污染源与传播途径分析及风险评估[J]. 中国食品工业, 2023(21):82−85. [CHEN Y J. Analysis and risk assessment of sources and transmission pathways of food microbial contamination[J] China Food Industry, 2023(21):82−85.] CHEN Y J. Analysis and risk assessment of sources and transmission pathways of food microbial contamination[J] China Food Industry, 2023（21）: 82−85.
[4]	金锋. 新型食品加工技术对食品营养的影响[J]. 中国食品工业,2023(15):106−107,110. [JIN F. The impact of new food processing technologies on food nutrition[J]. China Food Industry,2023(15):106−107,110.] JIN F. The impact of new food processing technologies on food nutrition[J]. China Food Industry, 2023（15）: 106−107,110.
[5]	王潇栋, 孔阳芷, 张艳玲, 等. 杀菌技术的作用机制及在食品领域中的应用[J]. 中国酿造,2022,41(2):1−8. [WANG X D, KONG Y Z, ZHANG Y L, et al. Mechanism of action of sterilization technology and its application in food field[J]. China Brewing,2022,41(2):1−8.] doi: 10.11882/j.issn.0254-5071.2022.02.001 WANG X D, KONG Y Z, ZHANG Y L, et al. Mechanism of action of sterilization technology and its application in food field[J]. China Brewing, 2022, 41（2）: 1−8. doi: 10.11882/j.issn.0254-5071.2022.02.001
[6]	ZHENG Q M, AIELLO A, CHOI Y S, et al. 3D printed photoreactor with immobilized graphitic carbon nitride:A sustainable platform for solar water purification[J]. Journal of Hazardous Materials,2020,399:123097. doi: 10.1016/j.jhazmat.2020.123097
[7]	DENG Y, LI Z, TANG R, et al. What will happen when microorganisms ''meet'' photocatalysts and photocatalysis?[J]. Environmental Science:Nano,2020,7(3):702−723.
[8]	GUO Q, ZHOU C Y, MA Z B, et al. Fundamentals of TiO₂ photocatalysis:Concepts, mechanisms, and challenges[J]. Adv Mater,2019,31(50):e1901997. doi: 10.1002/adma.201901997
[9]	DONG J Q, ZHANG Y, HUSSAIN M I, et al. G-C₃N₄:Properties, pore modifications, and photocatalytic applications[J]. Nanomaterials (Basel),2021,12(1):121. doi: 10.3390/nano12010121
[10]	YUE L, ZHENG M H, KHAN I M, et al. Chlorin e6 conjugated chitosan as an efficient photoantimicrobial agent[J]. International Journal of Biological Macromolecules,2021,183:1309−1316. doi: 10.1016/j.ijbiomac.2021.05.085
[11]	倪永升. 纳米石墨相氮化碳基食品包装膜的制备及杀菌机制研究[D]. 咸阳:西北农林科技大学, 2023. [NI Y S. Preparation and bactericidal mechanism of nano graphite phase carbon nitride based food packaging film[D]. Xianyang:Northwest A&F University, 2023.] NI Y S. Preparation and bactericidal mechanism of nano graphite phase carbon nitride based food packaging film[D]. Xianyang: Northwest A&F University, 2023.
[12]	杨欧, 张晓湘, 徐小涵, 等. 抗氧化型壳聚糖/大豆蛋白复合食用膜的制备与应用[J]. 食品工业科技, 2024, 45(6):210−218. [YANG O, ZHANG X X, XU X H, et al. Preparation and application of antioxidant chitosan/soy protein composite edible film [J] Science and Technology of Food Industry, 2024, 45(6):210−218.] YANG O, ZHANG X X, XU X H, et al. Preparation and application of antioxidant chitosan/soy protein composite edible film [J] Science and Technology of Food Industry, 2024, 45（6）: 210−218.
[13]	LI Z H, BAI H, WEI J L, et al. One-step synthesis of melamine-sponge functionalized carbon nitride for excellent water sterilization via photogenerated holes and photothermal conversion[J]. Journal of Colloid and Interface Science,2022,610:893−904. doi: 10.1016/j.jcis.2021.11.126
[14]	ABDElHACK M E, ELSSAADONY M T, SHAFI M E, et al. Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications:A review[J]. International Journal of Biological Macromolecules,2020,164:2726−2744. doi: 10.1016/j.ijbiomac.2020.08.153
[15]	MONTASER A S, WASSEL A R, ALSHAYEA O N. Synthesis, characterization and antimicrobial activity of Schiff bases from chitosan and salicylaldehyde/TiO₂ nanocomposite membrane [J]. International Journal of Biological Macromolecules,2018,124:802−809.
[16]	BIAN C H, WANG Y Y, YI Y Y, et al. Enhanced photocatalytic activity of S-doped graphitic carbon nitride hollow microspheres:Synergistic effect, high-concentration antibiotic elimination and antibacterial behavior[J]. Journal of Colloid and Interface Science,2023,643:256−266. doi: 10.1016/j.jcis.2023.04.034
[17]	QU B, LUO Y C. Chitosan-based hydrogel beads:Preparations, modifications and applications in food and agriculture sectors - A review[J]. Int J Biol Macromol,2020,152:437−448. doi: 10.1016/j.ijbiomac.2020.02.240
[18]	CAI L, WEI X, FEMG H, et al. Antimicrobial mechanisms of g-C₃N₄ nanosheets against the oomycetes Phytophthora capsici:Disrupting metabolism and membrane structures and inhibiting vegetative and reproductive growth[J]. Journal of Hazardous Materials,2021,417:126121. doi: 10.1016/j.jhazmat.2021.126121
[19]	刘骁, 孟茜, 张明莉, 等. 等离子体活化水对腐败希瓦氏菌杀菌效果及机理[J]. 食品科学,2023,44(9):25−31. [LIU X, MENG X, ZHANG M L, et al. The bactericidal effect and mechanism of plasma activated water on spoilage Shigella[J]. Food Science,2023,44(9):25−31.] doi: 10.7506/spkx1002-6630-20220520-264 LIU X, MENG X, ZHANG M L, et al. The bactericidal effect and mechanism of plasma activated water on spoilage Shigella[J]. Food Science, 2023, 44（9）: 25−31. doi: 10.7506/spkx1002-6630-20220520-264
[20]	WANG Y X, MALKKMES M J, JIANG C, et al. Antibacterial mechanism and transcriptome analysis of ultra-small gold nanoclusters as an alternative of harmful antibiotics against Gram-negative bacteria[J]. Journal of Hazardous Materials,2021,416:126236. doi: 10.1016/j.jhazmat.2021.126236
[21]	PRIYADARSHI R, RHIM J-W. Chitosan-based biodegradable functional films for food packaging applications[J]. Innovative Food Science & Emerging Technologies, 2020, 62: 102346.
[22]	赵鹏程. 壳聚糖衍生物氮化碳可见光催化灭活抗性菌及去除抗性基因的研究[D]. 合肥:合肥工业大学, 2022. [ZHAO P C. Research on visible light catalyzed inactivation of resistant bacteria and removal of resistance genes by chitosan derivative nitrogen doped carbon [D]. Hefei:Hefei University of Technology, 2022.] ZHAO P C. Research on visible light catalyzed inactivation of resistant bacteria and removal of resistance genes by chitosan derivative nitrogen doped carbon [D]. Hefei: Hefei University of Technology, 2022.
[23]	PRASEETJA P K, GODWIN M A, ALSALHI M S, et al. Porous chitosan-infused graphitic carbon nitride nanosheets for potential microbicidal and photo-catalytic efficacies[J]. International Journal of Biological Macromolecules,2023,238:124120. doi: 10.1016/j.ijbiomac.2023.124120
[24]	ZHAO C, YAN Q, WANG S, et al. Regenerable g-C(3)N(4)-chitosan beads with enhanced photocatalytic activity and stability[J]. RSC Adv,2018,8(48):27516−27524. doi: 10.1039/C8RA04293D
[25]	CESARI A B, PAULUCCI N S, BIASUTTI M A, et al. Changes in the lipid composition of Bradyrhizobium cell envelope reveal a rapid response to water deficit involving lysophosphatidylethanolamine synthesis from phosphatidylethanolamine in outer membrane[J]. Research in Microbiology,2018,169(6):303−312. doi: 10.1016/j.resmic.2018.05.008
[26]	YUAN L, FAN L Y, DAI H C, et al. Multi-omics reveals the increased biofilm formation of Salmonella typhimurium M3 by the induction of tetracycline at sub-inhibitory concentrations[J]. Science of The Total Environment,2023,899:165695. doi: 10.1016/j.scitotenv.2023.165695
[27]	LUO W, WANG J Q, SUN L, et al. Metabolome analysis shows that ultrasound enhances the lethality of chlorine dioxide against Salmonella Typhimurium by disrupting its material and energy metabolism[J]. Food Research International,2022,162:112135. doi: 10.1016/j.foodres.2022.112135
[28]	JAUREGUIBERRY M S, TRICERRI M A, SANCHEZ S A, et al. Role of plasma membrane lipid composition on cellular homeostasis:Learning from cell line models expressing fatty acid desaturases[J]. Acta Biochimica et Biophysica Sinica,2014,46(4):273−282. doi: 10.1093/abbs/gmt155
[29]	BLEIJERVELD O B, BROUWERS J F H M, VAANDRAGER A B, et al. The CDP-ethanolamine pathway and phosphatidylserine decarboxylation generate different phosphatidylethanolamine Molecular Species[J]. Journal of Biological Chemistry,2007,282(39):28362−28372. doi: 10.1074/jbc.M703786200
[30]	GAO Y, SHABALINA I G, BRAZ G R F, et al. Establishing the potency of N-acyl amino acids versus conventional fatty acids as thermogenic uncouplers in cells and mitochondria from different tissues[J]. Biochimica et Biophysica Acta (BBA)- Bioenergetics,2022,1863(4):148542. doi: 10.1016/j.bbabio.2022.148542
[31]	HANN R M, EVANS J E, MCCLUER R H, et al. Gangliosides in membranes from torpedo electric organ[J]. Lipids,1996,31(6):627−33. doi: 10.1007/BF02523833
[32]	WANG Y, GAO X F, YANG H S. Integrated metabolomics of “big six” Escherichia coli on pea sprouts to organic acid treatments[J]. Food Research International,2022,157:111354. doi: 10.1016/j.foodres.2022.111354
[33]	JOUHET J, MARECHAL E, BALDAN B, et al. Phosphate deprivation induces transfer of DGDG galactolipid from chloroplast to mitochondria[J]. J Cell Biol,2004,167(5):863−874. doi: 10.1083/jcb.200407022
[34]	CHEN X, WENG M F, LAN M J, et al. Superior antibacterial activity of sulfur-doped g-C₃N₄ nanosheets dispersed by Tetrastigma hemsleyanum Diels & Gilg's polysaccharides-3 solution[J]. International Journal of Biological Macromolecules,2021,168:453−463. doi: 10.1016/j.ijbiomac.2020.11.155
[35]	XU J S, DANG D K, TRAN V T, et al. Liquid-phase exfoliation of graphene in organic solvents with addition of naphthalene[J]. Journal of Colloid and Interface Science,2014,418:37. doi: 10.1016/j.jcis.2013.12.009
[36]	PENG S Y, LI Y J, HUANG M J, et al. Metabolomics reveals that CAF-derived lipids promote colorectal cancer peritoneal metastasis by enhancing membrane fluidity[J]. International Journal of Biological Sciences,2022,18(5):1912−1932. doi: 10.7150/ijbs.68484
[37]	CAI F Y, JIN S X, CHEN G J. The effect of lipid metabolism on CD4⁺ T Cells[J]. Mediators Inflamm,2021,2021:6634532.

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Science and Technology of Food Industry

Effects of Carbon Nitride Combined with Chitosan on Lipid Metabolism of Salmonella Typhimurium

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