Evaluation of Uncertainty in Determination of Four Organophosphorus Pesticide Residues in Fresh Tea Leaves by Gas Chromatography

LIU Tengfei; ZHANG Li; YANG Daifeng; ZHU Song; ZHOU Fengjie

doi:10.13386/j.issn1002-0306.2022040006

Volume 44 Issue 1

Dec. 2022

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Science and Technology of Food Industry > 2023 > 44(1): 323-331. > DOI: 10.13386/j.issn1002-0306.2022040006

LIU Tengfei, ZHANG Li, YANG Daifeng, et al. Evaluation of Uncertainty in Determination of Four Organophosphorus Pesticide Residues in Fresh Tea Leaves by Gas Chromatography[J]. Science and Technology of Food Industry, 2023, 44(1): 323−331. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040006.

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Evaluation of Uncertainty in Determination of Four Organophosphorus Pesticide Residues in Fresh Tea Leaves by Gas Chromatography

1.
Jiangsu Taihu Area Institute of Agricultural Sciences, Suzhou 215105, China
2.
Center for Food Safety and Nutrition, Suzhou Vocational University, Suzhou 215104, China
3.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China
4.
Suzhou Agricultural Products Safety and Quality Inspection Center, Suzhou 215128, China

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Received Date: April 01, 2022
Available Online: November 06, 2022

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Abstract

Abstract

Aim to evaluate the uncertain factors which may influence the accuracy of detection of pesticide residues in fresh tea leaves, a case study of dichlorvos, dimethoate, malathion and fenitrothion residues in fresh tea leaves was carried out using gas chromatography with external standard method. According to the relevant standard and protocol, a mathematical model has been studied. The uncertainties created by the entire experimental process were evaluated systematically. Three A-type evaluation components, including reproducibility of determination, fitness of standard curve and recovery rate, and four B-type evaluation components, including preparation of standard solution, sample weighing and pretreatment, and the measurement of instrument were investigated. The results revealed that the expanded uncertainty of measured values of dichlorvos, dimethoate, malathion and fenitrothion in the spiked tea leaves were 0.021, 0.073, 0.036 and 0.023 mg/kg (k=2, confidence interval P=95%), while the theoretical content were 0.096, 0.39, 0.19 and 0.19 mg/kg, respectively. Preparation of standard solution and fitness of standard curve contributed to the major effect on uncertainty, which should be paid more attention for future determination. Our study provides a reference for the accuracy assessment of method in detection of four organophosphorus pesticide residues in fresh tea leaves.
- gas chromatography,
- fresh tea leaves,
- organophosphorus pesticides,
- uncertainty

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References

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Evaluation of Uncertainty in Determination of Four Organophosphorus Pesticide Residues in Fresh Tea Leaves by Gas Chromatography

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