Effect of Soft-shelled Turtle Peptide on the Lifespan and Antioxidant Activities of <i>Drosophila melanogaster</i>

SHI Jinyuan; ZHONG Hao; WANG Qianqian; LUO Xianliang; WANG Jing; YU Peng; FENG Fengqin

doi:10.13386/j.issn1002-0306.2020070211

Volume 42 Issue 11

May 2021

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Science and Technology of Food Industry > 2021 > 42(11): 321-327. > DOI: 10.13386/j.issn1002-0306.2020070211

SHI Jinyuan, ZHONG Hao, WANG Qianqian, et al. Effect of Soft-shelled Turtle Peptide on the Lifespan and Antioxidant Activities of Drosophila melanogaster [J]. Science and Technology of Food Industry, 2021, 42(11): 321−327. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2020070211.

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Effect of Soft-shelled Turtle Peptide on the Lifespan and Antioxidant Activities of Drosophila melanogaster

SHI Jinyuan^{1, 2, 3,},
ZHONG Hao^{1, 2, 3},
WANG Qianqian^{1, 2, 3},
LUO Xianliang^{1, 2, 3},
WANG Jing^{1, 2, 3},
YU Peng⁴,
FENG Fengqin^{1, 2, 3, ,}

1.
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
2.
Key Laboratory of Agro-Products Postharvest Handling Ministry of Agriculture, Hangzhou 310058, China
3.
Ningbo Research Institute, Zhejiang University, Ningbo 315100, China
4.
Yuyao Lengjiang Turtle Industry, Ningbo 315400, China

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Received Date: July 16, 2020
Available Online: April 01, 2021

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Abstract

Abstract

Objective: To investigate the effect of soft-shelled turtle peptide (STP) on the lifespan and antioxidant activities of Drosophila melanogaster. Methods: The soft-shelled turtle peptide was prepared by enzymatic digestion. The molecular mass distribution and amino acid composition of STP were measured.Male and female Drosophila melanogaster were fed on a diet supplemented with different doses of soft-shelled turtle peptide (0.2%, 0.4%, 0.8%). The antioxidant effect of soft-shelled turtle peptide was evaluated by analyzing the survival rate and measuring the activity of superoxide dismutase (SOD), catalase (CAT) and the content of malondialdehyde (MDA) of D. melanogaster. Results: The results showed that STP were mainly constituted with peptides which had relative molecular weight between 180 and 500 Da. Moreover, STP were rich in proline, lysine and leucine. After treating with STP, compared with the control group, mean life span in high-dose group were significantly increased by 10.80% (P<0.05) and 14.01% (P<0.01) for female and male flies. Maximum mean life-span in high-dose group were significantly increased by 6.35% (P<0.01) and 13.22% (P<0.01) for female and male flies. The activity of SOD in high-dose group were significantly increased by 28.40% (P<0.01) and 15.08% (P<0.01) for female and male flies. The activity of CAT was increased by 33.50% (P<0.05) and 38.05% (P<0.01) while the content of MDA was decreased by 33.33% (P<0.01) and 34.62% (P<0.01), respectively. Conclusion: Soft-shelled turtle peptide can increase a series of antioxidant enzyme activities and decrease lipid peroxidation to prolong life span of D. melanogaster. Thus, STP may have potential anti-aging effect.
- soft-shelled turtle peptide,
- molecular mass distribution,
- amino acid composition,
- lifespan,
- antioxidant activities

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Science and Technology of Food Industry

Effect of Soft-shelled Turtle Peptide on the Lifespan and Antioxidant Activities of Drosophila melanogaster

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