Breeding of High Performance <i>Metarhizium anisopliae</i> Strain by ARTP/UV Mutagenesis

HUANG Yu; Nimazhaxi; XUE Zhenglian; ZHANG Guoqiang

doi:10.13386/j.issn1002-0306.2020060082

Volume 42 Issue 4

Feb. 2021

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Science and Technology of Food Industry > 2021 > 42(4): 60-64,70. > DOI: 10.13386/j.issn1002-0306.2020060082

HUANG Yu, Nimazhaxi, XUE Zhenglian, ZHANG Guoqiang. Breeding of High Performance Metarhizium anisopliae Strain by ARTP/UV Mutagenesis[J]. Science and Technology of Food Industry, 2021, 42(4): 60-64,70. DOI: 10.13386/j.issn1002-0306.2020060082

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Breeding of High Performance Metarhizium anisopliae Strain by ARTP/UV Mutagenesis

1. College of Biological and Chemical Engineering, Anhui Polytechnic University, Wuhu 241000, China;
2. Institute of Resources and Environment, Tibet Academy of Agricultural and Animal Husbandry Sciences, Lhasa 850000, China

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Received Date: June 07, 2020
Available Online: March 01, 2021

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Abstract

After atmospheric and room temperature plasma(ARTP)and ultraviolet(UV)compound mutation,a high-performance strains of Metarhizium Anisopliae was selected. By comparing the speed of sporulation and the size of growth diameter,selected with the ability of anti-ultraviolet and virulence as the re-screening index,and tested for heat resistance and genetic stability,a good strain AU34 with high sporulation,UV tolerance,strong virulence and stable genetic was selected at the time of ARTP 40 s and UV 120 s. The sporulation time of the strain was faster than the original strain,and the sporulation amount was 1.63±0.22(10⁸ cell/cm²),which was 63.81% higher than the original strain. After 5 min of UV irradiation,the survival rate of AU34 was 3.18%,which was more resistant to UV irradiation than the original strain. The toxicity of AU34 to Plutella xylostella L. was high. The adjusted mortality rate was 85.71% and LT₅₀ was 6.12 days. Compared with the original strain,the heat resistance of AU34 was also improved. After subculture for 6 generations,the sporulation of strain AU34 had no obvious change and had good genetic stability.
- Metarhizium anisopliae,
- atmospheric and room temperature plasma(ARTP),
- ultraviolet mutagenesis,
- compound mutation,
- screening,
- genetic stability

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