Extraction,Purification and Antioxidant Activity of Total Flavonoids from Mulberry Leaves

In this study,single factor test and response surface analysis were used to optimize the extraction process of total flavonoids from mulberry leaves,and the antioxidant activity of total flavonoids from mulberry leaves was determined by hydrogen peroxide scavenging test. The results showed that the best extraction process parameters were:Ethanol concentration 80%,ratio of material to liquid 1:30 g/mL,ultrasonic time 75 min,ultrasonic temperature 40 ℃,and the extraction rate was 2.7%.The content of total flavonoids in mulberry leaves extract increased from 12.3% to 33.8% after being treated with AB-8 macroporous resin. The antioxidant results showed that the antioxidant capacity of total flavonoids in mulberry leaves were higher than that of ascorbic acid,and it could be a potential natural antioxidant.