Optimization of Enzymatic Hydrolysis Process of Calcium Binding Peptides from Shrimp Shell by Response Surface Methodology

Calcium binding peptides from shrimp shell was prepared by enzymatic extraction in this research.Based on degree of hydrolysis and calcium binding capacity,single factor experiments of pH,enzyme dosage,time,temperature,substrate concentration were researched. And the enzymatic condition was optimized by response surface methodology(RSM). The results showed that,alcalase was used for hydrolysis and the optimum conditions were as follows:pH10.2,enzyme dosage 4100 U/g,time 5.5 h,temperature 55 ℃ and substrate concentration 10%. In condition,the degree of hydrolysis was 16.33%±0.27% and calcium binding capacity was(1.954±0.020) mg/mL.