Simultaneous Determination of Citrus Red 2 and SudanⅠ~Ⅳ Dyes in Foodstuff by Ultra Performance Liquid Chromatography-tandem Mass Spectrometry

A method for determination of Citrus Red 2 and SudanⅠ~Ⅳ in food by ultra performance liquid chromatography-tandem mass spectrometry was established. The samples were extracted with acetonitrile sodium chloride system and purified by NH₂ column. The separation was carried out by ultra performance liquid chromatography(UPLC). The target compounds were detected by MS/MS system with electrospray ionization(ESI)under multiple reaction monitoring(MRM)mode. Results showed that,the calibration curves of 5 illegal dyes showed good linearity in 2~40 ng/mL with determination coefficients 0.998. The average recoveries ranged from 80.7% to 114% at three spiking levels with RSD<8.6%. The limit of detection(LOD)and the limit of quantitation(LOQ)of Citrus Red 2 and SudanⅠ~Ⅳ in five matrics were 5.0 and 10.0 μg/kg,respectively. This rapid and sensitive method could be applied for quantitative determination of Citrus Red 2 and SudanⅠ~Ⅳ in foodstuff.