Screening of the mimic epitopes of deoxynivalenol from cyclo-heptapeptide library

Screening of mimetic epitopes of deoxynivalenol by using random Ph.D-7 library.DON monoclonal antibody as the target molecule, and Ph.D-7 library had been panned for 4 times. The positive phage was screened out and tested by ELISAspecificity.The activity of 30 phage monoclones was determined by indirect ELISA and indirect competitive ELISA. It showed that 28 phages could combined with DON monoclonal antibody and 22 of them were able to compete with DON standards for binding to DON m Abs.The results of DNA sequencing showed sequence of 21 was PFPNHPY, and the another one was TPWTQHL.The competition curve of corresponding phage and DON toxin standard product showed inhibition curve for the linear range of NO.8 phage was 0.0292⁰.636 μg/mL and IC₅₀ was 0.169 μg/mL, and NO.4 phage was 0.0124⁰.271 μg/mL, IC₅₀ was 0.058 μg/mL.TPWTQHL and PFPNHPY were obtained from Ph.D-7 phage display peptide library.The competitive inhibition curves of phage and DON were established respectively.Preliminary validation shows that the DON toxin standard can be replaced by two DON mimotopes and can be established DON immunological non-toxic detection techniques.