Isolation and identification of thermoactinomycete from Moutai-flavor vinasse in Moutai region
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Graphical Abstract
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Abstract
High temperature resistant strains were isolated from Moutai-flavor vinasse, numbered as DF. The individual morphology and colony morphology were observed, and the 16 S rDNA gene was sequenced, and starch, cellulose and protein degradation ability of high temperature resistant strains were studied. The results showed that colony of the strain DF was dry and tight, and the inside of the medium was dark yellow, and the surface had a white and opaque powder. The growth temperature ranged from 35 to 60 ℃.The optimum growth temperature was 55 ℃.The strain DF was identified preliminarily as Thermomonospora chromogena by 16 S rDNA gene sequences analysis and phylogenetic tree analysis. The strain had some degradation ability of starch, cellulose and protein.It could be used as bacteria agent of lees degradation.
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