Study on production of feruloyl oligosaccharides by compound enzymatic hydrolysis from maize bran

Production of feruloyl oligosaccharides by enzymatic hydrolysis form maize bran was investigated in this paper. In order to explore the reason that influenced the yield of feruloyl oligosaccharides, maize bran was hydrolyzed by different enzymes and the cell wall of maize bran was also determined.In the end, the optimal enzyme hydrolysis condition was optimized by single factor and response surface experiment.Results showed that the mixture of cellulase and xylanase could improve maize bran cell wall dissolving, and could improve the yield of feruloyl oligosaccharides.The optimal condition for FOs production was mixture enzyme addition of 15.7 g/L, which was consist with 70% cellulase addition, and the maize bran material addition was at 100 g/L, and hydrolysis for 1 h, under this condition, the yield of FOs was 1008.43 μmol/L.This result provides theoretical basis for FOs production by compound enzymatic hydrolysis from maize bran.