Delphinidin sensitizes anti-tumor effect to HER-2+breast cancer cells by combining with 3-MA

Objective: To research the effect of delphinidin( Dp) combines with 3- Methyladnine( 3- MA),inhibitor of autophagy,to HER( human epidermal growth factor receptor-2,HER-2) positive breast cancer cell MDA- MB-453.Methods:HER-2+breast cancer cells MDA- MB-453 were treated by Dp.Proliferation of MDA- MB-453 cells was detected by CCK-8.Fluorescence dot assay and Weston Blot were used to identify autophagy in breast cancer cells. Weston blot was used to assay condition of autophagy inhibition of 3- MA to MDA- MB- 453 cell and CCK- 8 tests combination effect of Dp and 3- MA to MDA- MB-453 cell.Results: Dp suppressed proliferation effectively and induced autophagy in dose- dependent manner in MDA- MB-453 cells.However,3- MA could restrain the autophagy,which sensitized anti- proliferation of Dp to MDA- MB- 453 cell.Conclusion: Combination of Dp and 3- MA which suppresses autophagy can sensitize anti- proliferation effect of HER-2+breast cancer cell MDA- MB-453.