Optimization of cellulase extraction of Sparassis crispa polysaccharides using response surface methodology

In order to get higher extraction efficiency,the polysaccharides from Sparassis crispa were extracted using cellulase.A single- factor study was designed to investigate the effects of different factors on the yield of polysarccharides,such as enzymatic addition,enzymatic hydrolysis temperature,enzymatic hydrolysis time,liquid /material ratio and p H value.Based on the results of single- factor assay,several factors were chosen to be further optimized by response surface methodology. The optimal process parameters were showd as follows: enzymatic addition of 1.3%,extraction temperature of 40 ℃,hydrolysis time of 2.5 hours,the liquid / solid ratio of 42 m L / g,and p H5.5.Under these above conditions,the content of polysaccharides could be up to 22.90% ± 0.26%.