Isolation and preliminary characterization of lipases from fungi in extreme environments

From 76 soil samples,collected in South Africa,99 basophilic filamentous fungi strains and 51 thermophilic filamentous fungi strains were screened.Two measuring methods,including olive oil emulsification and p- NPP,had been used to test the activity of lipase and three filamentous fungi strains had been selected for hydrolyzing special substrate. They were F4- 173,F4- 262 and F8- 67,identified as Aspergillus fumigatus,Penicillium verrucosum,Penicillium chrysogenum by the method of ITS,respectively.The result showed that the strain F4-173 could hydrolyze canola oil and its optimal temperature and p H were 40 ℃ and 8.0,the strain F4- 262 could hydrolyze p- nrophenyl laurate and the optimal temperature and p H of the enzyme were 40 ℃ and 8.0,the strain F8-67 could only hydrolyze soybean oil and its optimal temperature and p H value for lipase activity were 50 ℃ and6.5 respectively.