Determination of deltamethrin residues in honey by HPLC coupled with ultrasound-assisted dispersive liquid-liquid microextraction

A method of HPLC combined with ultrasound-assisted dispersive liquid-liquid microextraction was used for the determination of deltamethrin in honey. The honey sample was extracted with mixed solution of acetonitrile∶water(1∶4,v/v). After the chloroform extraction,ultrasound and centrifugation,sedimentary facies were determined by HPLC. Deltamethrin of 10~1000 μg/kg ranged with good linearity. The correlation coefficient was 0.9998,and the enrichment factor was up to 600. When the spiked concentration of honey samples came to 10,50,100 μg/kg,the recovery rates measured by standard addition method ranged from 82.6% to 97.3%,with the relative standard deviation(RSD) between 2.1% and 3.8%. The detection limit of deltamethrin in honey samples was 8 μg/kg,and the limit of quantification was 40 μg/kg. This method was simple and accurate,with high efficiency of extraction and little consumption of organic solvent. It could be successfully applied to the determination of deltamethrin residues in honey.