Separation, purification and antioxidant activity of polyphenols from Sanguisorba officinalis

Through comparing the adsorption and desorption rates of 7 kinds of macroporous resin to polyphenols, the XAD-8 resin was selected to purify polyphenols of Sanguisorba officinalis. The optimal purification conditions were the sample concentration of 2.5 mg / m L, p H5.0, the adsorption equilibrium of 3 h, ethanol elution volume fraction of 60%, the sample flow rate of 1.0 m L / min, elution flow rate 1.5 m L / min. After purification of Sanguisorba officinalis polyphenols, the polyphenol purity were increased from 20.79% to 62.97%. Polyphenols had strong antioxidant capacity, the scavenging hydroxyl free radical activities and reducing activities of it were superior to VC, the IC50 values of hydroxyl radical and DPPH radical were respectively 0.179 mg / m L and 0.691 mg / m L.