Optimization of the fermentation conditions for siderophores from Synechococcus sp.PCC 7002 by response surface methodology

In order to improve the yield of siderophores from Synechococcus sp. PCC 7002,response surface methodology( RSM) was used to optimize the fermentation conditions. On the basis of single factor experiment,Box- Behnken RSM was employed to investigate the four factors( temperature,p H,carbon sources,nitrogen sources) on the yield of siderophores.The optimum culture conditions were as follows: the temperature was 31.7 ℃,p H was 8.28,the amount of glucose was 14.47 g / L,the amount of NH4 Cl was 0.19 g / L.Under these conditions the relative amount of siderophores from Synechococcus sp.PCC 7002 was 95.42% after 15 days fermentation while the other group without optimizing was just 64.53%.The relative amount of siderophores was increased by 47.87% after optimizing. The culture conditions obtained by RSM were effective and reliable,the yield of siderophores improved significantly.