Study on some enzyme property of feruloyl esterase from wheat malt

Taking de- starched wheat bran as substrate,the enzyme activity of feruloyl esterase in wheat malt were confirmed and some enzyme property was discussed as follows: the optimal temperature and p H were 35 ℃ and6.0,the enzyme activity were 0.126 U / g and 0.125 U / g respectively.The survival rate was above 97.9% after 120 min incubating between 25~ 35 ℃. The enzyme was basically inactivated by incubating for 120 min at 60 ℃ and the survival rate was only 4.6%. The enzyme was inactivated in 80,20,10 min respectively by incubating at 65,70,75 ℃. Feruloyl esterase had high stability when incubated in p H 5.5 ~ 6.0. Ca2 +was significantly increased the enzyme activity,Hg+and EDTA showed significantly inhibitory effect on the enzyme activity.