Optimization of salt-soluble protein from oratoria by response surface methodology

To optimize the fast extraction condition for the oratosquilla oratoria salt-soluble protein,the best assisted extraction methods was obtained on the basis of single-factor investigation. The optimize extraction conditions were as follow:extraction solution p H of 6.36,extraction solution concentration of Na Cl was 0.11 mol/L,material to water ratio at 1∶3,and magnetic stirring extraction-duration of 24 min. The salt-soluble protein content could reach 22.46 mg/g,and the theory predicted value(22.71 mg/g) differs by 0.25 mg/g.