Optimization of the fermentation conditions for transglutaminase by response surface methodology

In the paper,Streptomyces mobaraensis was used as the original strain. Based on the single-factor tests,significant factors such as initial p H,rotating speed,medium volume and cultivation temperature were selected to optimize the flake conditions for TG fermentation by the Box-Behnken design with 4 factors and 3levels experiment,when TG activity was designated as the response value. Results suggested that the optimal conditions for TG fermentation were 78 m L/250 m L of medium volume and 150 r/min of rotation speed at 30 ℃with initial p H7.0 for 96 h cultivation,under which the transglutaminase activity was up to(1.41±0.02) U/m L.