Study on detection method of salmonella using aptamer- conjugated microbeads

Objective: To investigate the feasibility of aptamer- conjugated microbeads for the detection of salmonella.Methods: Amino- terminal aptamer probes were covalently attached onto the surface of microbeads.Fluorescence from microbeads was detected as signal source, and parameters were optimized which may affect detection of the target.Results: Salmonella were detected in six of 20 aquatic product samples by this method, and the detection rate was 30%. Conclusion: The aptamer- conjugated microbeads can be used for high throughput and accurate detection of salmonella.