Comparison of inhibitory effect of sodium silicate at different concentrations and corresponding p H on Trichothecium roseum in vitro and in vivo
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Graphical Abstract
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Abstract
The inhibitory effect were assayed on spore germination and colony growth of T.roseum treated with sodium silicate at different concentration and corresponding p H.The lesion diameter was determined in apple fruits inoculated with pores treated with 100 mmol / L sodium silicate and corresponding p H12.60 for 3, 6 and 9h.Morphology of spores treated with 100 mmol / L sodium silicate and corresponding p H12.60 was observed by SEM.The results showed that sodium silicate at different concentration and corresponding p H significantly inhibited the spore germination and colony growth. However, sodium silicate treatments showed more effective than corresponding p H treatments ( p < 0.05) . Apples inoculated with spores treated with sodium silicate had a smaller lesion diameter when compared with the control; however, no difference was found in lesion diameter treated with corresponding p H and the control. Spores treated with sodium silicate appeared rough, significant shrinkage, damaged structure and contents exudate. The treatment with corresponding p H also caused the shrinkage of spores, but the structure was not damaged. It was suggested that sodium silicate treatment caused more severe damage than corresponding p H treatment against T.roseum.
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