Silver nitrate-silica gel column chromatography purification linolenic acid in the walnut oil

The fatty acids of walnut oil were separated primarily by urea adduction method, then the linolenic acid of walnut oil was further purified by silver nitrate-silica gel column chromatography. In the silver silica gel column formed by 20 g silica gel and 2g Ag NO3, join in the ratio was 1∶15 with adsorbent after urea inclusion enrichment of fatty acids, 100 m L 8% acetone-petroleum, underd the elution flow rate of 0.6m L/min, the content of linoleic acid in walnut oil increased from the original 8.7% to 95.9%. This suggests that the silver nitrate-silica gel column chromatography is a kind of high efficient separation method of linolenic acid.