Antioxidant activity of polyphenolics and flavonoid extracted from wild apple (Malus sieversii)

Twenty different lines of wild apple (Malus sieversii) collected from different places in Xinjiang, China were used as research objects. Total total polyphenol content was measured using the Folin- Ciocalteau method. Total flavonoid content was measured by spectrophotometer. Total antioxidant capacities were analyzed by ABTS assay, DPPH assay, and iron ( Ⅲ) to iron ( Ⅱ) reducing power assay, and the correlation between the various indicators was measured with Pearson correlation coefficient. It was found that HDM-23 had the highest total polyphenol content (4714.33μmol gallic acid/100 g fresh matter) , and XY-31 contained the highest amount of total flavonoid content (2452.84 mg rutin/100 g fresh matter) . The present study implied that total polyphenols contribute to the antioxidant properties of the wild apple (p<0.01) and total flavonoid was an important phenolic compound in the wild apple varieties investigated.