Determination of citrinin in grain by dispersive solid- phase extraction and HPLC

Objective: To establish a method to determine the content of citrinin in grain. Method: The samples were extracted with 60% ethanol,and cleaned up by dispersive solid- phase extraction with C18 dispersant sorbent and ready for HPLC determination.Results: A standard curve equation was built as follows: Y = 3.13 × 10- 7X-5.59 × 10- 2with linear correlation coefficient of 0.9999 and RSD was 4.51%.The average recovery range was 87.7% ~96.4%.The citrinin content range of the samples was 0.116 ~ 0.385μg / g.Conclusion: This method was sample and accurate,which could be used for detecting citrinin in grain.