Camellia oleifera seed oil recovery from emulsion through alkaline demulsificatication

The demulsification of the emulsion formed during aqueous enzymatic extraction of camellia oleifera seed oil by alkaline was studied.Demulsification rate was taken as index.Based on single- factor experiments,the main factors were optimized with response surface methodology( RSM). By employing RSM design programmed,the optimal parameters were generated as follows: p H = 9.0,temperature 60℃,time 45 min and demulsification rate was90.15%.Fluorescence microscopy technology was applied to observe the change of oil droplets before and after alkaline demulsificatication,respectively. The comparision found that alkaline demulsificatication oil droplets aggregated significantly,grease easier to release.