Development of PCR- DHPLC detection method for five types of Staphylococcal enterotoxin genes

Five pairs of primers were designed and synthesized for five Staphylococcal enterotoxins genes( SEA,SEB,SEC,SED,SEE). The PCR- DHPLC detection methods for five Staphylococcal enterotoxins genes were established after optimizing of the annealing temperature and DNA template amount.The sensitivity and specificity of the detection method were then determined. The results showed that the PCR- DHPLC method could detect specifically of Staphylococcal enterotoxins genes with detection limit of 100 cfu / m L. The PCR- DHPLC detection method had some advantages,such as rapid,accuracy and high throughput. The PCR- DHPLC method had fine application value to detecting Staphylococcal enterotoxins in import and export foods.