Expression and functional analysis of an alternative sigma factor,lsig B of Streptomyces rimous in E.coli

In this research,the gene lsig B encoding regulatory factors from Streptomyces rimosus was heterologous expressed in E.coli.After the identification of Lsig B protein by SDS- PAGE and Western blotting,recombinant E.coli was cultured under a variety of environment stress. The results showed that the expression of Lsig B significantly enhanced the stress resistance of recombinant strains to heat,ehanol,oxygen and salt stress,together with antibiotic pressure. Therefore,the main function of Lsig B respoding to environmental stress was demonstrated.Meanwhile,the study was to lay foundation for researching the resistance function of Lsig B in Streptomyces rimosus.