Study on extraction process of total flavoniods and its antioxidant effect from Chloranthus henryi

Objective: To study the extraction technology of total flavonoids from Chloranthus henryi and its antioxidant activity.Methods: The optimum condition for extracting total flavonoids from Chloranthus henryi by microwave-assisted was obtained through orthogonal experiments.And the antioxidant activity of the extract was evaluated by spectrophotometry on its ability of scavenging hydroxyl free radical (·OH) and 1- diphenyl-2- picrylhydrazy free radical ( DPPH·) , total reducing power.Results: The optimum procedure for microwave- assisted extraction of total flavonoids were ethanol concentration of 60%, ratio of material to solvent of 1 ∶ 30, microwave power of 300 W, microwave time of 15 min and that the flavonoids yield was up to 6.11%.The scavenging rates of total flavonoids from Chloranthus henryi against hydroxyl free radical and DPPH free radical were found to be 100% and 76.2%, and the extract also had a strong total reducing power at a certain concentration range ( 0.2 ~ 0.8mg /mL) . Conclusion: The results showed that the extract from Chloranthus henryi was active for oxidation resistance, and it showed good application future for this plant.