The development of high cellulase production mutant from Trichoderma Viride and study on the condition of enzyme production

The cellulase production strain T. viride 8140 was treated with UV and EMS. The mutant strain T.viride8140UEUE4-80 was showed to have a higher cellulase activity than others and the Hc of the mutant reached 2.1. After several passages its CMCase and FDAase were stabilized at 21.05U/mL and 2.4U/mL. The conditions of fermentation were studied and the optimal culture medium was decided. Under the optimal culture medium, the CMCase and FPAase of the mutant separately reached 22.5, 2.52U/mL.