Optimization of process parameters for total dietary fiber from leaves of Toona Sinensis with compound enzyme method by response surface methodology

The method of single factor test and response surface analysis was adopted to optimize the enzymatic extraction of total dietary fiber ( TDF) from leaves of Toona sinensis with cellulose and amylase ( 1∶1) .The results showed that enzyme dosage 0.5%, enzymolysis time 2.0h, temperature 61℃, and pH6.1. The TDF extracting rate was 74.01% under these optimum conditions with low levels of impurities.The physiological activation was good, the holding water capacity and swelling property were 6.50 g /g and 4.50 mL /g respectively.