Experimental study on antitumor effects of five Alkylphenol from wheat bran and its preliminary mechanism

Objective: The aim of this study was to observe the inhibitory effect of five alkylphenols from wheat bran in vitro, and explore its mechanism.Method: The proliferation and IC50 of five compounds on LoVo, HepG2, MDA-MB-231 cancer cell lines were assayed by MTT method in vitro.Flow cytometry was applied to analyze the effect of cell cycle with different concentrations of 5- heptadecylresorcinol in LoVo cancer cell lines. Result: The inhibition effects of 5- heptadecylresorcinol on all three tumor cell lines were significant, and 5- nonadecylresorcinol took second place.The other compounds had less toxicity to all three tumor cell lines than 5- nonadecylresorcinol.5-Heptadecylresorcinol could inhibit the proliferation of LoVo cells during G2/M period.Conclusion: The antitumor activity of five alkylphenols may be related to its length of side chains. Our results showed that5-heptadecylresorcinol possess good effects of inhibiting tumor cell proliferation in vitro.It may be a potential anticancer leading compound and need study further.