Study on debittering of papain soluble collagen peptide from Grass carp scale

In this paper, papain soluble collagen peptide ( PSCP) was used as research object, which extracted from grass carp scale, to study PSCP recovery rate under nanofiltration membranes with different molecular weight cut off ( MWCO) .Orthogonal experiment ( L9 ( 34) ) was used to research the influence of β-cyclodextrin dosage, bath time and bath temperature on fishy bitterness and PSCP recovery rate. Finally, content and proportion of PSCP amino acid were analyzed. The results showed that, recovery rate ( 16.71%) was lowest when the nanofiltration membrane MWCO was about 400~600u, suggesting that the molecular weight of PSCP might between 300~600u.The optimal embedding conditions were cyclodextrin amount of 4%, bath temperature of 70℃, bath time of 40 min.Under this condition, the best deodorization effects were achieved with PSCP recovery rate was 92.21% and sensory score was 9.30.The analysis results of PSCP amino acid showed that there were 16 kinds of amino acid content of more than 1% in PSCP, total content of 99.07% in total amino acid, in which the hydroxyproline ( Hyp) content was higher than 8%.The method and process parameters optimized could provide a basis for industrial production.