Optimization acid-heating method for extracting chitosan from waste Aspergillus niger by using response surface methodology
-
WEI Zi-hao1,2,3,
-
CHEN Ji-hong2,3,
-
ZHANG Zhen1,
-
HU Wei2,3,
-
ZHANG Yang1,
-
LIU Jing2,3,
-
LI Wen-jian2,3
-
College of Food Science and Engineering, Gansu Agricultural University
-
Institute of Modern Physics, Chinese Academy of Sciences
-
Key Laboratory of Microbial Resources Exploition and Application, Gansu Province
More Information
-
-
- Received Date:
September 17, 2013
-
-
Abstract
This article discussed the practicability of acid-heating method which could break Aspergillus niger's cell walls in order to extract chitosan.Based on the single factor test analysis, the effects of three variables, which including cell wall breaking temperature, cell wall breaking time, and concentration of hydrochloric acid on the technology of breaking Aspergillus niger cell wall and the optimum process were evaluated by Box-Behnken central composite experimental design and response surface methodology analysis.The results indicated that cell wall breaking temperature of 100℃, cell wall breaking time of 1.6h, and concentration of hydrochloric acid of 2.1% were the optimum condition.The experimental values of chitosan yield were 10.87%, and the relative error with the theoretical value 11.06% was 1.72%. Therefore, the chitosan extraction from Aspergillus niger optimized by response surface methodology was very reliable.
-
-
References
| [1] |
付云霄, 张桂, 赵国群.壳聚糖在果汁澄清中的应用[J].食品工程, 2011 (1) :15-17.
|
| [2] |
蒋挺大.壳聚糖[M].北京:化学工业出版社, 2006:72-86.
|
| [3] |
蔡俊, 杜予民, 杨建红, 等.酶法提取柠檬酸废菌体中壳聚糖的研究[J].食品与发酵工业, 2005, 31 (12) :39-41.
|
| [4] |
陈雪梅, 肖文胜, 邓祥义, 等.从柠檬酸厂废黑曲霉菌丝体中提取壳聚糖的工艺研究[J].化学与程, 2005 (8) :43-45.
|
| [5] |
赵继伦, 王红林.利用柠檬酸废菌体制备壳聚糖的工艺研究[J].工业微生物, 1999, 29 (2) :33-37.
|
| [6] |
曹健, 代养勇, 李浪, 等.黑曲霉电解法制备甲壳素的研究[J].食品科学, 2006, 27 (2) :186-192.
|
| [7] |
Jun Cai, Jianhong Yang, Yumin Du, et al.Enzymatic preparation of chitosan from the waste Aspergillus niger mycelium of citric acid production plant[J].Carbohydrate Polymers, 2006, 64:151-157.
|
| [8] |
李光, 方晓兰, 蔡志辉, 等.利用黑曲霉废菌丝体提取壳聚糖的研究[J].岳阳职业技术学院学报, 2012, 27 (6) :80-82.
|
| [9] |
张建, 游玲, 邓鹜远, 等.柠檬酸废渣制取壳聚糖中微波辅助脱蛋白工艺研究[J].食品工业科技, 2010, 31 (6) :276-278.
|
| [10] |
胡伟, 陈积红, 张珍, 等.重离子辐照柠檬酸菌株的诱变选育[J].辐射研究与辐射工艺学报, 2012, 30 (1) :54-57.
|
| [11] |
曾哲灵, 范镭, 印培民, 等.从米根霉中提取壳聚糖的初步研究[J].中国酿造, 2008 (20) :16-19.
|
| [12] |
王传芬.从废菌丝体中提取壳聚糖的研究[J].中国酿造, 2009 (10) :123-126.
|
-
DownLoad: