Study on purification of recombinant antihypertensive peptides VLPVPR with macroporous adsorption resin

To separate and purify peptide VLPVPR, the absorption capability and purification effect of VLPVPR on macroporous adsorption resin ( DA201-C Ⅱ, D4020 and HPD100B) were evaluated. Adsorption and desorption experiments were carried out to screen the suitable macroporous adsorption resin for VLPVPR.DA201-CⅡ was the best macroporous adsorption resin for purification of VLPVPR.The optimization conditions were pH9.8, feeding rate 2mL /min, feeding volume 100mL, ethanol concertration 70%, elute rate 2.7mL /min and elute volume 75mL. The recovery ratio was 96.9% and the purity ratio was 27.4%.