Optimization of purification process of angiotensin converting enzyme inhibitor peptide VLPVPR with macroporous resin

To separate and purify angiotensin converting enzyme inhibitor peptide VLPVPR, the absorption capability and purification effect of VLPVPR on macroporous resin were evaluated. Adsorption and desorption experiments were carried out to screen the suitable macroporous resin for VLPVPR. HPD100B was the best macroporous resin for purification of VLPVPR. The optimization condition were temperature 20℃, pH9.8, feeding rate 3mL/ (cm2·min) , feeding volume 180mL, ethanol concertration 60%, elute rate 0.25mL/ (cm2·min) and elute volume 45mL. The desorption ratio was 93.1%, the purity ratio was 28.8% and the inhibition ratio (IC50) of the ACE was 4.1μmol/L.