Optimization of synthesis of lentinan-Iron (Ⅲ) complex by response surface methodology

Based on the single factor tests, the effects of mass ratio between lentinan and catalyst sodium citrate, pH reaction temperature on iron combination rate of lentinan- Fe ( Ⅲ) were designed using Box-Behnken to assess the combination rate of iron (Ⅲ) . A prediction model was established. The optimal synthesis conditions were mass ratio between lentinan and catalyst sodium citrate of 1.8, pH8.0, reaction temperature of 68.70℃, iron combination rate of lentinan-Fe (Ⅲ) was 11.69%. It was generally accepted that the response surface method was scientific and optimum for the synthesis of lentinan-Iron (Ⅲ) complex.