LIU Rong, JIANG Yuan-song, YANG Wei-wei, WANG Zhen-yu. Secondary purification and composition analysis of polyphenols from Pinus sylvestris L.barks[J]. Science and Technology of Food Industry, 2014, (03): 113-118. DOI: 10.13386/j.issn1002-0306.2014.03.023
Citation: LIU Rong, JIANG Yuan-song, YANG Wei-wei, WANG Zhen-yu. Secondary purification and composition analysis of polyphenols from Pinus sylvestris L.barks[J]. Science and Technology of Food Industry, 2014, (03): 113-118. DOI: 10.13386/j.issn1002-0306.2014.03.023

Secondary purification and composition analysis of polyphenols from Pinus sylvestris L.barks

More Information
  • Received Date: July 23, 2013
  • The best resin was selected from nine kinds of macroporous resins by adsorption and resolution rate as indicators.Pinus sylvestris L.barks polyphenol was secondary purified with the best resin using purity and recovery rate as index.Finally, the composition of secondary purified polyphenol was preliminary analyzed with HPLC-DAD- ESI-MS method. The results showed that the macroporous resin X- 5 was the best resin and 60% ethanol was suitable for eluent. The optimum condition of secondary purification was that sample solution concentration was 4.0mg /mL, chromatographic column diameter hight ratio was 1 ∶ 30, sample solution volume was 60mL, eluting speed was 2BV/h.Under this condition, the purity of Pinus sylvestris L.barks polyphenol increased from 62.06% ± 1.67% to 72.99% ± 0.98%, the recovery rate was 87.26% ± 2.42%. The main composition of secondary purification product were: procyanidins trimer, procyanidin dimmer type B2, 5-galloylquinic acid, epicatechin and ellagic acid.
  • loading
  • [1]
    林樱姬, 赵萍, 王雅.植物多酚的提取方法和生物活性研究进展[J].陕西农业科学, 2009, 55 (6) :105-107.
    [2]
    赵海田, 王振宇, 程翠林, 等.松多酚类活性物质抗氧化构效关系与作用机制研究进展[J].食品工业科技, 2012, 33 (2) :458-461.
    [3]
    T Y Hsu, S C Sheu, E T Liaw, et al.Anti-oxidant activity and effect of Pinus morrisonicola Hay.on the survival of leukemia cell line U937[J].Phytomedicine, 2005, 12 (9) :663-669.
    [4]
    李健, 杨昌鹏, 李群梅.植物多酚的应用研究进展[J].广西轻工业, 2008 (12) :1-3.
    [5]
    Ali Liazid, Monica Schwarz, Rosa M Varela, et al.Evaluation of various extraction techniques for obtaining bioactive extracts from pine seeds[J].Food and Bioproducts Processing, 2010, 88 (2-3) :247-252.
    [6]
    M Pinelo, M Rubilar, J Sineiro, et al.Extraction of antioxidant phenolics from almond hulls (Prunes amygdalus) and pine sawdust (Pines pinaster) [J].Food Chemistry, 2004, 85 (2) :267-273.
    [7]
    徐青, 陆莹莹, 辛健美, 等.大孔树脂吸附分离海芦笋中黄酮类化合物工艺[J].食品科学, 2011, 32 (2) :115-119.
    [8]
    杜晓.大孔树脂技术在中草药分离纯化中的应用[J].青海大学学报, 2012 (1) :62-65.
    [9]
    熊何健, 吴国宏.大孔树脂分离纯化葡萄多酚的研究[J].食品研究与开发, 2007, 128 (11) :74-77.
    [10]
    周文亮, 孙蕴哲, 唐星.大孔树脂纯化柿叶总黄酮工艺考察[J].中国药剂学杂志, 2008, 6 (5) :276-282.
    [11]
    H Li, J Liu, D Li, et al.Study on Separation and Purification of Genistein in the Soybean Residue Using Macroporous Resin Adsorption[J].Industrial&Engineering Chemistry Research, 2012, 51 (1) :44-49.
    [12]
    X L Chang, D Wang, B Y Chen, et al.Adsorption and Desorption Properties of Macroporous Resins for Anthocyanins from the Calyx Extract of Roselle (Hibiscus sabdariffa L.) [J].Journal of Agricultural and Food Chemistry, 2012, 60 (9) :2368-2376.
    [13]
    刘荣, 何娇, 王振宇.大孔树脂对樟子松树皮多酚纯化工艺的研究[J].食品工业科技, 2013, 34 (11) :201-205.
    [14]
    赵玉红, 翟亚楠, 王振宇.樟子松树皮中松多酚的提取工艺研究及提取方法比较[J].食品工业科技, 2013, 34 (4) :304-309.
    [15]
    李波, 包怡红, 王振宇.大孔树脂纯化红松松球鳞片多酚及其抗氧化活性研究[J].食品工业科技, 2012, 33 (22) :251-255.
    [16]
    白夺龙, 杨开华.大孔吸附树脂分离纯化技术及应用[J].海峡药学, 2007, 92 (9) :96-99.
    [17]
    刘子放, 王振江, 唐翠明, 等.桑根花青素的大孔树脂分离纯化[J].广东蚕业, 2008, 4 (4) :31-33.
    [18]
    何炳林, 黄文强.离子交换与吸附树脂[M].上海:上海科技教育出版社, 1995.
    [19]
    朱静, 陆晶晶, 袁其朋.大孔吸附树脂对石榴皮多酚的分离纯化[J].食品科技, 2010, 35 (l) :188-193.
    [20]
    耿中华, 秦卫东, 马利华, 等.梨皮多酚的提取工艺优化的研究[J].食品工艺科技, 2009, 30 (12) :233-238.
    [21]
    Laura M Bystrom, Betty A LEWIS, Dan L Brown, et al.Characterisation of phenolics by LC-UV/Vis, LC-MS/MS and sugers by GC in Melicoccus bijugatus Jacq.‘Montgomery’fruits[J].Food Chemistry, 2008, 111:1017-1024.
    [22]
    Toshihiko Shoji, Saeko Masumoto, Nina Moriichi, et al.Apple (Malus pumila) procyanidins fractionated according to the degree of polymerization using normal-phase chromatography and characterized by HPLC-ESI/MS and MALDI-TOF/MS[J].Journal of chromatography A, 2006, 1102:206-213.
    [23]
    Maarit K, Jyrki L, Vladimir O, et al.Analysis of procyanidins in pine bark with reversed-phase and normal-phase highperformance liquid chromatography-electrospray ionization mass spectrometry[J].Analytica ChimicaActa, 2004, 522:105-112.
    [24]
    Oischlacer C, Regos I, Zeller F J.et al.Identification of galloylated propelargonidins andprocyanidins in buckwheat grain and quantification of rutin and flavanols homostylous hybrids originating from F.esculentum×F.homotropicum[J].Phytochemistry, 2008, 69:1389-1397.
    [25]
    Laurent Hollecker, Maurizio Pinna, Gioraia Filippino, et al.Simultaneous determination of polyphenolic compounds in red and white grapes grown in Sardinia by high performance liquid chromatography-electron spray ionization-mass spectrometry[J].Journal of Chromatography A, 2009 (2) :10-12.
    [26]
    Dongmei Wang, Jiali Liu, Aiqing Miao, et al.HPLC-DADESI-MS/MS analysis of polyphenols and purine alkaloidsin leaves of 22 tea cultivars in China[J].Journal of Food Composition and Analysis, 2008 (21) :361-369.
    [27]
    Marin Prodanoy, Ignacio Garrido, Visitacion Vacas, et al.Ultrafiltration as alternative purification procedure for the characterization of low and high molecular-mass phenolics from almond skins[J].Analytica Chimica Acta, 2008, 609:241-251.
    [28]
    M C Díaz-García, J M Obón, M R Castellar, et al.Quantification by UHPLC of total individual polyphenols in fruit juices[J].Food Chemistry, 2013 (138) :938-949.

Catalog

    Article Metrics

    Article views (156) PDF downloads (248) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return