Optimization of process conditions for the extraction of chondroitin sulfate by response surface methodology

The chicken sternal cartilage was used as the raw material and the chondroitin sulfate extraction yield as the target to withdraw the method by ultrasonic assisted alkali-enzymatic. Firstly, the ranges of all affecting factors were determined by single factor experiment and Box-Behnken center combination experimental design used to set up two times multiterm mathematical model. The result indicated that the data results significantly and the optimum conditions of ultrasonic assisted alkali-enzymatic were obtained as follows :the alkali concentration was 4%, enzyme dosage was 1.4g/L, ratio material to solvent was 1000 ∶5923 (g/mL) , and the chondroitin sulfate extraction yield was 23.94%. Determined the extraction of substances through the UV spectrum detection of chondroitin sulfate.