Purification and antioxidative activity of polysaccharides from cold-extracting apple peel

Apple polysaccharides were extracted from cold- extracting apple peel. The isolation and purification of apple polysaccharides were studied by changing the polarity of eluent and the antioxidative activity of polysaeeharides were analysed. The results indicated that apple polysaccharides of apple peel through DE- 52 cellulose column chromatography obtained three main elution peaks. The main elution components of water, 0.3mol /L NaCl and 0.9mol /L NaCl were further pured by Sephadex G-200 medium and were named as APPS- H 2 O、APPS-0.3mol /L and APPS-0.9mol /L.The antioxidation experiments showed that all of three polysaccharides had the reducing powers and the scavenging activity of·OH, DPPH·and O- 2 ·, but the antioxidative activities were lower than the crude polysaccharides and V C.