LIU Bo, WU Ying-long, ZHANG Xia, HUANG Ya-li. Optimization of solid state fermentation medium of Monascus M2 for xylanase production by response surface analysis[J]. Science and Technology of Food Industry, 2014, (01): 254-258. DOI: 10.13386/j.issn1002-0306.2014.01.036
Citation: LIU Bo, WU Ying-long, ZHANG Xia, HUANG Ya-li. Optimization of solid state fermentation medium of Monascus M2 for xylanase production by response surface analysis[J]. Science and Technology of Food Industry, 2014, (01): 254-258. DOI: 10.13386/j.issn1002-0306.2014.01.036

Optimization of solid state fermentation medium of Monascus M2 for xylanase production by response surface analysis

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  • Received Date: July 14, 2013
  • The influence of carbon sources, nitrogen source, mineral salt on xylanase activity producted by Monascus M2 in the solid state fermentation was studied.On the base of single factors experiments, response surface analysis was applied to optimize the solid state fermentation medium of Monascus M2 for xylanase production.The quadratic regression analysis was applied to get the optimal level of main factors ( corn meal, beef extract, K 2 HPO 4) .Finally determined the optimal medium as follows: corn meal 1.90g, beef extract 0.55g, K 2 HPO 4 0.10g.Under these optimal conditions, the predicted and experimental production of the activity of xylanase was high up to 1550.62 U /g and 1545.38 U /g, respectively.
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  • [1]
    孙雷, 朱孝霖, 李环, 等.基因工程菌1020耐热木聚糖酶的纯化与性质[J].食品科学, 2006, 27 (9) :76-78.
    [2]
    Coughlan M P, Hazlewood G P.β-1, 4-D-xylan-degrading enzyme system:biochemistry, molecularbiology and applications[J].Biotechnol Apply Bioche, 1993, 17:259.
    [3]
    吴萍, 李正鹏, 何庆元, 等.金针菇固态发酵产木聚糖酶的纯化及其性质研究[J].药用生物技术, 2012, 19 (1) :31-34.
    [4]
    符丹丹, 李爱江, 谢慧, 等.宇佐美曲霉木聚糖酶的纯化及其性质[J].食品科学, 2006, 27 (2) :116-120.
    [5]
    江正强.微生物木聚糖酶的生产及其在食品工业中应用的研究进展[J].中国食品学报, 2005, 5 (1) :1-8.
    [6]
    孙振涛, 赵祥颖, 刘建军, 等.微生物木聚糖酶及其应用[J].生物技术, 2007, 17 (2) :93-97.
    [7]
    Fengxue Xin, Jianzhong He.Characterization of a thermostable xylanase from a newly isolated Kluyvera species and its application for biobutanol production[J].Bioresource Technology, 2013, 135:309-315.
    [8]
    Sella L, Gazzetti K, Faoro F, et al.A Fusarium graminearum xylanase expressed during wheat infection is a necrot-iczing factor but is not essential for virulence[J].Plant Physiology and Biohemistry, 2013, 64:1-10.
    [9]
    Kumar T V.Satyanarayana.Thermo-alkali-stable xylanase of a novel polyextremophilic Bacillus halodurans TSEV1 and its application in biobleaching[J].International Biodeterioration&Biodegradation, 2012, 75:139-145.
    [10]
    暴立娟, 宋庆凤, 李杰.重组木聚糖酶的安全高效表达与应用研究[J].食品工业科技, 2011 (1) :156-161.
    [11]
    Sanjay, Asish Mandal, Pradeep K, et al.Production of xylanase byimmobilized Trichoderma reesei SAF3 in Ca-alginate beads[J].Journal of Industrial Microbiology&Biotechnology, 2008, 35 (4) :245-249.
    [12]
    李市场, 白爱枝, 余增亮, 等.离子束诱变木聚糖酶产生菌 (Aspergillus niger A3) 筛选方法的比较研究[J].激光微生物学报, 2003, 12 (2) :149-152.
    [13]
    Chun-Han Ko, Chung-Hung Tsai, Jenn Tu, et al.Identification of Paenibacillus sp.2S-6 and application of its xylanase on bio-bleaching.[J].International Biodeterioration&Biodegradation, 2011, 65 (2) :334-339.
    [14]
    Bajaj B K, Manhas K.Production and characterization of xylanase from Bacillus licheniformis P11 (C) with potential for fruit juice and bakery industry[J].Biocatalysis and Agricultural Biotechnology, 2011, 1 (4) :330-337.
    [15]
    Zhengqiang Jiang, Qianqian Cong, Qiaojuan Yan, et al.Characterisation of a thermostable xylanase from Chaetomium sp.and its application in Chinese steamed bread[J].Food Chemistry, 2010, 120 (2) :457-462.
    [16]
    曹云鹤, 陈小玲, 贺平丽, 等.硫色曲霉木聚糖酶基因xyn A的克隆、表达及酶学性质分析[J].生物技术通讯, 2006, 17 (6) :878-881.
    [17]
    江正强, 杨绍青, 李里特, 等.嗜热拟青霉固体发酵产木聚糖酶的纯化和性质[J].工业微生物, 2006, 36 (3) :1-4.
    [18]
    徐子钧, 李剑, 梁凤来, 等.利用SAS软件优化L-乳酸发酵培养基[J].微生物学通报, 2004, 31 (3) :85-87.
    [19]
    王晓青, 曾洪梅, 石义萍.农用抗生素2-16高产菌株选育及发酵优化组合研究[J].微生物学通报, 2005, 32 (6) :7-11.
    [20]
    高鹏飞, 李妍, 赵文静, 等.益生菌Lactobacilluscasei Zhang增殖培养基的优化[J].微生物学通报, 2008, 35 (4) :623-628.
    [21]
    Suny, Wangzf, Wujh, et al.Optimising enzymatic macerationin pretreatment of carrot juice concentrate by response surface methodology[J].International Journal of Food Science and Technology, 2006, 41 (9) :1082-1089.
    [22]
    Miller GL.Use of dinitrosalicylic acid reagent for determination of reducing sugar[J].Anal Chem, 1959, 31:426-427.
    [23]
    Lowry O H, Rosebrough N J, Farr A L, et al.Protein measurement with the Folin phenol reagent[J].The Journal of Biological Chemistry, 1951, 193:265-275.
    [24]
    Palaniswamy M, Pradeep B V, Sathya R, et al.Isolation, identification and screening ofpotential xylanolytic enzyme from litter degrading fungi[J].African Journal of Biotechnology, 2008, 7 (11) :1978-1982.
    [25]
    代义, 吕淑霞, 林英, 等.高产木聚糖酶菌株筛选、鉴定及产酶条件的研究[J].生物技术, 2008, 18 (2) :70-73.
    [26]
    周薇薇, 尹亚辉, 赵长新.响应面法优化黑曲霉固态发酵产木聚糖酶工艺[J].大连工业大学学报, 2013, 32 (3) :176-179.

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