Optimization of activation condition of prothrombin from horse blood by using response surface methodology
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College of Chemistry and Chemical Engineering, Scientific Research Department, Xinjiang Normal University
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Department of Life, College of Life Science, Xinjiang Normal University
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College of Food Sciences, Xinjiang Agricultural University
More Information
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- Received Date:
April 23, 2013
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Abstract
Based on single factor experiment, three main factors were screened out, including activation time, activation temperature, and concentration of Ca2 +. Optimization of these factors by using response surface methodology, the results showed that the optimum activation conditions of prothrombin were as follows:activation time 2.8h, temperature 32℃, concentration of Ca+20.11mol/L. Under these conditions, the specific activity of thrombin was 51.16U/mg.
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References
| [1] |
欧伶, 宋聿文.猪凝血酶原的制备[J].中国医药工业, 1997, 28 (10) :435-438.
|
| [2] |
庄秀华.局部止血药-凝血酶简介[J].海峡药学, 2002, 14 (5) :93-94.
|
| [3] |
Carminita Frost, Ryno Naude.Purification and characterization of ostrich prothrombin[J].The International journal of Biochemistry&Cell Biology, 2000 (32) :1151-1159.
|
| [4] |
Sarah E Michaud, louise Z Wang.Purification of salmon thrombin and its potential as an alternative to mammalian thrombins in fibrin sealants[J].Thrombosis Rsearch, 2002, 107:245-254.
|
| [5] |
韩国才.我国马业的新趋势[J].中国动物保健, 2005 (10) :26.
|
| [6] |
李征, 熊祥政.人凝血酶的制备[J].华西药学杂志, 1998, 13 (4) :253-255.
|
| [7] |
Macgregor.United states patent:US, 5907032[P].1999-5-25.
|
| [8] |
乌云达来, 孙振钧, 王冲, 等.牛血铜锌超氧化物岐化酶规模化生产工艺研究[J].家畜生态学报, 2006, 27 (5) :46-52.
|
| [9] |
钟峻, 印文考, 林缨, 等.高纯度人凝血酶的制备[J].中国医学科学院学报, 1995, 17 (1) :36-40.
|
| [10] |
Montgomery.Design and analysis of experiments[M].6th Edition.傅珏生译.北京:人民邮电出版社, 2009 (1) :347-389.
|
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