Research of degradation of tartaric acid by Aspergillus niger F1 by using the quadratic orthogonal rotational combination design

A quadratic orthogonal rotation combination design was used to explore the effects of incubation temperature, inoculation amount of spore suspension, medium volume, tartaric acid concentration of Aspergillus niger F1 on degradation rate of tartaric acid. A regression equation was established using Design Expert 7.1 software. The optimum tartaric acid - degrading conditions were incubation temperature of 34.7℃, inoculation amount of 4.3% , medium volume 163mL, tartaric acid concentration 9.3g /L.Under such conditions, its degradation rate was 98.3%.