Separation and purification of shikimic acid from pine needles with ion exchange resin

The adsorption and desorption rates of 4 types of adsorption resins towards shikimic acid from the Pine Needles were investigated.Among the studied ion exchange resin, 331 anion exchange resin was the most suitable to purify crude shikimic acid from the Pine Needles.When 4BV of 1.5mg /mL sample was flowed through 331 anion exchange resin column at a speed of 2mL /min, the adsorption of the 331 anion exchange resin reached dynamic equilibrium.The adsorbed shikimic acid could be completely eluted with 8BV of 2% sodium hydroxide solution at 4mL / min.After the purification, the purity of shikimic acid was increased from 25% to 90% , resulting in a purification yield of 90.9%.