Process optimization on cellulose enzymatic hydrolysis of Perilla frutescens stalk

The process optimization on cellulose enzymatic hydrolysis of Perilla frutescens stalks was studied. The content of reducing sugar was determined by DNS method to evaluate the enzymatic hydrolysis.After analysis on hydrolysis time, enzyme content and pH value respectively, the optimized hydrolysis conditions were obtained by orthogonal experiments.Experimental results showed that the optimum condition was: reaction time 24h, enzyme content 720U, pH5.0.Under the optimum condition, the efficiency of enzymatic hydrolysis could reach 72.42%.