Study on the extraction process of oat antifreeze protein by vacuum infiltration centrifugation
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Graphical Abstract
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Abstract
The extraction process of oat antifreeze protein was investigated. The optimal extraction process was established as following:buffer:50mmol/L PBS, soaking time:60min, vacuum time:60min, vacuum degree:0. 35MPa, centrifugal speed:3000r/min, centrifugation time:25min. The results of DSC indicated the protein obtained had a significant thermal hysteresis activity with the THA of 2. 07℃.
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