Determination of 5-hydroxymethylfurfural in fruit juice concentrate and fruit fructose by HPLC

A HPLC method was established for the determination of 5-hydroxymethylfurfural (5-HMF) in fruit juice concentrate and fruit fructose. The sample was diluted with water, separated by using Inetrsil ODS-3 Cl8 (250mm×4. 6mm, 5μm) chromatographic column. The method showed that a good linearity within the range of 1. 0 ~ 25. 0mg/L (r=9998) . The LOD of 5-HMF was 0. 2mg/kg, the average recoveries from 82. 2% to 103. 3%, the relative standard deviation (RSD) from 0. 62% to 1. 25%. The HPLC method is quick, accurate, sensitive and suitable for determining the content of 5-HMF in fruit juice concentrate.